State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, 210008, P. R. China.
University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
J Microbiol. 2018 Nov;56(11):783-789. doi: 10.1007/s12275-018-8178-0. Epub 2018 Oct 24.
Two Gram-stain negative halophilic strains, designated as LM2 and LM4, were isolated from Lake LongmuCo on Tibetan Plateau. These two strains were aerobic, catalaseand oxidase-positive, nonmotile and rod-shaped organisms. Phylogenetic analysis based on 16S rRNA gene sequences indicated that LM2 and LM4 belong to the genus Roseovarius, with Roseovarius tolerans EL-172 (97.3% and 97.4% 16S rRNA gene sequence similarity, respectively) and Roseovarius azorensis SSW084 (95.5% and 95.6% 16S rRNA gene sequence similarity, respectively) as their closest neighbors. Q-10 was the sole respiratory quinone of these two strains. The major fatty acids were Cω7c/Cω6c, C, C cyclo ω8c, and 11-methyl Cω7c. The polar lipids included phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phospholipid of unknown structure containing glucosamine, and unidentified aminolipid. The DNA G + C content was between 64.2 and 64.5 mol%. DNA-DNA hybridization showed 96.7% relatedness between LM2 and LM4, 24.9% relatedness between LM2 and R. tolerans EL-172, and 36.3% relatedness between LM4 and R. tolerans EL-172. Based on phylogenetic analysis, DNA-DNA hybridization, a range of physiological and biochemical characteristics, LM2 and LM4 belong to the same species and were clearly distinguished from the type strains of the genus Roseovarius. It was evident that LM2 and LM4 could be classified as a novel species of the genus Roseovarius, for which the name Roseovarius tibetensis sp. nov. is proposed. The type strain is LM2 (= CGMCC 1.16230 = KCTC 62028).
从青藏高原龙木错湖分离到两株革兰氏阴性嗜盐菌，分别命名为 LM2 和 LM4。这两株菌为需氧、接触酶和氧化酶阳性、非运动和杆状的生物体。基于 16S rRNA 基因序列的系统发育分析表明，LM2 和 LM4 属于玫瑰杆菌属，与 Roseovarius tolerans EL-172（分别为 97.3%和 97.4%的 16S rRNA 基因序列相似性）和 Roseovarius azorensis SSW084（分别为 95.5%和 95.6%的 16S rRNA 基因序列相似性）最为接近。这两株菌的唯一呼吸醌为 Q-10。主要脂肪酸为 Cω7c/Cω6c、C16:0、C cyclo ω8c 和 11-甲基 Cω7c。极性脂包括磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、含有葡萄糖胺的未知结构磷脂和未鉴定的胺脂。DNA G+C 含量在 64.2%至 64.5 mol%之间。DNA-DNA 杂交显示 LM2 和 LM4 之间的相关性为 96.7%，LM2 和 R. tolerans EL-172 之间的相关性为 24.9%，LM4 和 R. tolerans EL-172 之间的相关性为 36.3%。基于系统发育分析、DNA-DNA 杂交、一系列生理生化特征，LM2 和 LM4 属于同一物种，与玫瑰杆菌属的模式菌株明显不同。因此，LM2 和 LM4 可被分类为玫瑰杆菌属的一个新种，命名为 Roseovarius tibetensis sp. nov. 。模式菌株为 LM2（=CGMCC 1.16230=KCTC 62028）。
