Wright Debbie E, Colaco Susanna, Colaco Camilo, Stevenson Philip G
Division of Virology, Department of Pathology, University of Cambridge, UK.
Immunobiology Ltd, Babraham Research Campus, Cambridge, UK.
J Gen Virol. 2009 Nov;90(Pt 11):2592-2603. doi: 10.1099/vir.0.014266-0. Epub 2009 Jul 22.
Antibody is an important antiviral defence. However, it is considered to do little against human gamma-herpesviruses, which establish predominantly latent infections regulated by T cells. One limitation on analysing these infections has been that latency is already well-established at clinical presentation; early infection may still be accessible to antibody. Here, using murid herpesvirus-4 (MuHV-4), we tested the impact of adoptively transferred antibody on early gamma-herpesvirus infection. Immune sera and neutralizing and non-neutralizing monoclonal antibodies (mAbs) all reduced acute lytic MuHV-4 replication. The reductions, even by neutralizing mAbs, were largely or completely dependent on host IgG Fc receptors. Therefore, passive antibody can blunt acute gamma-herpesvirus lytic infection, and does this principally by IgG Fc-dependent functions rather than by neutralization.
抗体是一种重要的抗病毒防御机制。然而，人们认为它对人类γ-疱疹病毒作用甚微，这类病毒主要建立由T细胞调控的潜伏感染。分析这些感染的一个限制在于，在临床表现时潜伏状态已经确立；而早期感染可能仍可被抗体作用。在此，我们利用鼠疱疹病毒4型（MuHV-4），测试了过继转移抗体对早期γ-疱疹病毒感染的影响。免疫血清以及中和性和非中和性单克隆抗体（mAb）均能减少MuHV-4的急性裂解复制。即便中和性mAb所导致的复制减少，在很大程度上或完全依赖于宿主IgG Fc受体。因此，被动抗体可抑制急性γ-疱疹病毒裂解感染，且主要通过IgG Fc依赖性功能而非中和作用来实现。
