RealStar 基孔肯雅病毒实时逆转录聚合酶链反应试剂盒的性能
Performance of the RealStar Chikungunya virus real-time reverse transcription-PCR kit.
作者信息
Panning Marcus, Hess Markus, Fischer Waldemar, Grywna Klaus, Pfeffer Martin, Drosten Christian
机构信息
Institute of Virology, University of Bonn Medical Centre, Sigmund Freud-Str. 25, 53127 Bonn, Germany.
出版信息
J Clin Microbiol. 2009 Sep;47(9):3014-6. doi: 10.1128/JCM.01024-09. Epub 2009 Jul 22.
Abstract
A novel commercial Chikungunya virus real-time reverse transcription-PCR (RT-PCR) kit was evaluated on a comprehensive panel of original patient samples. The assay was 100% sensitive and specific in comparison to a published real-time RT-PCR. Viral loads from both assays were highly correlated. The kit proved to be suitable for routine use in patient care.
摘要
一种新型的商业化基孔肯雅病毒实时逆转录聚合酶链反应（RT-PCR）试剂盒在一组全面的原始患者样本上进行了评估。与已发表的实时RT-PCR相比，该检测方法的灵敏度和特异性均为100%。两种检测方法的病毒载量高度相关。该试剂盒被证明适用于患者护理的常规使用。
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