抗坏血酸在人皮肤成纤维细胞中的积累。

Ascorbic acid accumulation in human skin fibroblasts.

作者信息

Butler J D, Bergsten P, Welch R W, Levine M

机构信息

Human Genetics Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892.

出版信息

Am J Clin Nutr. 1991 Dec;54(6 Suppl):1144S-1146S. doi: 10.1093/ajcn/54.6.1144s.

DOI:10.1093/ajcn/54.6.1144s

PMID:1962561

Abstract

The transport and accumulation of ascorbic acid in normal human skin fibroblasts in culture was investigated by using high-performance liquid chromatographic separation and coulometric electrochemical detection. Results measured as picomole ascorbic acid per microgram cell protein were expressed in molar amounts after determining the volume of skin fibroblasts. Confluent fibroblasts contained undetectable amounts of ascorbic acid. On incubation with micromole per liter amounts of ascorbic acid in the medium, cells showed increasing uptake of ascorbic acid with time, accumulating a 15-fold excess in 3.5 h. Kinetic experiments suggested two transport mechanisms, a high-affinity and a low-affinity transport activity. Both transport activities were temperature sensitive and accumulated ascorbic acid against a concentration gradient.

摘要

采用高效液相色谱分离和库仑电化学检测法，对培养的正常人皮肤成纤维细胞中抗坏血酸的转运和积累进行了研究。以每微克细胞蛋白中抗坏血酸的皮摩尔数来衡量结果，在确定皮肤成纤维细胞的体积后，以摩尔量表示。汇合的成纤维细胞中抗坏血酸含量无法检测到。在培养基中加入每升微摩尔量的抗坏血酸进行孵育时，细胞对抗坏血酸的摄取随时间增加，在3.5小时内积累了15倍过量的抗坏血酸。动力学实验表明存在两种转运机制，即高亲和力和低亲和力转运活性。两种转运活性均对温度敏感，且能逆浓度梯度积累抗坏血酸。