Margolis S A, Ziegler R G, Helzlsouer K J
National Institute of Standards & Technology, Gaithersburg, MD 20899.
Am J Clin Nutr. 1991 Dec;54(6 Suppl):1315S-1318S. doi: 10.1093/ajcn/54.6.1315s.
Plasma supplemented with ascorbic acid was prepared; the stability of these samples was characterized and the accuracy of the supplementation was established. Studies on the accuracy, precision, and sources of methodological bias in the measurement of ascorbic acid were summarized. Measurements of the ratio of ascorbic acid to dehydroascorbic acid in clinical samples was evaluated and was shown to be relatively constant in plasma taken from blood stored at 12 degrees C for 6 h. These results imply that whole blood has the capacity to maintain a constant ascorbic-dehydroascorbic acid ratio and suggest that this ratio may be of physiological significance.
制备了添加抗坏血酸的血浆；对这些样品的稳定性进行了表征，并确定了添加的准确性。总结了抗坏血酸测量中准确性、精密度和方法学偏差来源的研究。评估了临床样品中抗坏血酸与脱氢抗坏血酸的比例测量，结果表明，在12摄氏度下储存6小时的血液所采集的血浆中，该比例相对恒定。这些结果表明全血有能力维持恒定的抗坏血酸 - 脱氢抗坏血酸比例，并表明该比例可能具有生理意义。
