Okamura M
Clin Chim Acta. 1980 May 9;103(3):259-68. doi: 10.1016/0009-8981(80)90144-8.
Various conditions for reducing L-dehydroascorbic acid were studied, using dithiothreitol. This led to an improved method for the separate determination of L-ascorbic acid and L-dehydroascorbic acid in blood plasma. The content of ascorbic acid was determined by an alpha,alpha'-dipyridyl method (A). Dehydroascorbic acid was reduced to ascorbic acid by incubation with dithiothreitol at room temperature for 10 min at pH 6.5 to 8.0. After removing the excess dithiothreitol with N-ethylmaleimide, total ascorbic acids, i.e. reduced plus original, were determined by the alpha,alpha'-dipyridyl method (B). The amount of dehydroascorbic acid could then be calculated from these results (B minus A).
使用二硫苏糖醇研究了还原L-脱氢抗坏血酸的各种条件。这产生了一种改进的方法，用于分别测定血浆中的L-抗坏血酸和L-脱氢抗坏血酸。抗坏血酸的含量通过α，α'-联吡啶法（A）测定。在室温下于pH 6.5至8.0用二硫苏糖醇孵育10分钟，将脱氢抗坏血酸还原为抗坏血酸。用N-乙基马来酰亚胺除去过量的二硫苏糖醇后，通过α，α'-联吡啶法（B）测定总抗坏血酸，即还原的加上原来的。然后可以从这些结果（B减去A）计算出脱氢抗坏血酸的量。
