Marascalco Philip J, Blair Harry C, Nieponice Alejandro, Robinson Lisa J, Kameneva Marina V
Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA.
ASAIO J. 2009 Sep-Oct;55(5):503-8. doi: 10.1097/MAT.0b013e3181b1840f.
We tested whether soluble viscoelastic drag-reducing polymers (DRPs), which modify blood flow in the macro- and microcirculation, affect host response to implanted biomaterials and control biodegradation and tissue ingrowth processes. Porous poly(L-lactate) (PLLA) implants, which are naturally hydrolyzed by foreign body giant cells, were used to evaluate differences in host response. Intravenous DRPs, high-molecular weight poly(ethylene oxide) (PEO) or poly(mannose) (PMNN), were given biweekly at 0.3-0.4 nM in saline (equivalent volumes of saline in controls) to rats with subcutaneous PLLA implants. After 7 weeks, there was no difference in weight gain or behavior between control and DRP-injected groups. Implanted PLLA scaffolds in controls were almost totally degraded and replaced by giant cell granulomas. On the contrary, PEO- or PMNN-treated animals retained a significant part of the implanted scaffold (p < 0.0001 vs. controls). The foreign body reaction was markedly decreased, and there was an increase in well-oriented collagen deposition within the implanted scaffold area in the animals treated with DRPs. The DRP-mediated effects observed in this study potentially reflect alteration in inflammatory events in response to implanted bioengineered materials, and, thus, warrant further investigation.
我们测试了可溶解的粘弹性减阻聚合物（DRP）是否会影响宿主对植入生物材料的反应，并控制生物降解和组织向内生长过程，这种聚合物可改变大循环和微循环中的血流。多孔聚-L-乳酸（PLLA）植入物会被异物巨细胞自然水解，我们用其评估宿主反应的差异。将静脉注射用DRP、高分子量聚环氧乙烷（PEO）或聚甘露糖（PMNN）以0.3-0.4 nM的浓度每两周一次溶于盐水中（对照组注射等量盐水），注射给皮下植入PLLA的大鼠。7周后，对照组和注射DRP组之间在体重增加或行为方面没有差异。对照组中植入的PLLA支架几乎完全降解，被巨细胞肉芽肿取代。相反，经PEO或PMNN处理的动物保留了很大一部分植入的支架（与对照组相比，p < 0.0001）。异物反应明显减轻，在接受DRP处理的动物中，植入支架区域内取向良好的胶原蛋白沉积有所增加。本研究中观察到的DRP介导的效应可能反映了对植入生物工程材料的炎症反应的改变，因此值得进一步研究。
