Boeris Valeria, Farruggia Beatriz, Romanini Diana, Picó Guillermo
Bioseparation Lab., Physical-Chemistry Department, Faculty of Biochemical and Pharmaceutical Sciences, National University of Rosario, FonCyT, CIUNR and CONICET, Suipacha 570, (S2002RLK) Rosario, Argentina.
Protein J. 2009 Jun;28(5):233-9. doi: 10.1007/s10930-009-9188-x.
Bovine serum albumin was selected as a model protein to study the molecular mechanism of interaction between flexible polymer with net negative electrical charge (polyvinylsulphonate and polyacrylic acid) and a non-charged polymer such as poly(ethylene) poly(propylene) oxide (molecular mass 8,400) by using spectroscopies techniques combination: fluorescence emission and circular dichroism. Polyvinylsulphonate and polyacrylic acid interact with the protein due to the coulombic interaction between positive charged protein groups such as amine of lysine and histidine. The poly(ethylene)-poly(propylene) oxide increased the hydrophobic microenvironment around the tryptophan residues. This polymer preserved the secondary and tertiary structure of the protein and did not induce any significant modification in the protein surface area exposed to the solvent.
选择牛血清白蛋白作为模型蛋白，通过荧光发射和圆二色性等光谱技术组合，研究带净负电荷的柔性聚合物（聚乙烯磺酸盐和聚丙烯酸）与不带电聚合物如聚（乙烯）聚（丙烯）氧化物（分子量8400）之间相互作用的分子机制。聚乙烯磺酸盐和聚丙烯酸与蛋白质相互作用是由于带正电荷的蛋白质基团（如赖氨酸和组氨酸的胺基）之间的库仑相互作用。聚（乙烯）-聚（丙烯）氧化物增加了色氨酸残基周围的疏水微环境。这种聚合物保留了蛋白质的二级和三级结构，并且没有在暴露于溶剂的蛋白质表面积上引起任何显著变化。
