Institute for Medical Research and Occupational Health, Zagreb, Croatia.
Med Mycol. 2010 Mar;48(2):298-302. doi: 10.1080/13693780903115402.
Farm and sawmill workers are exposed to high levels of allergenic fungi, such as Alternaria alternata, which are associated with respiratory diseases and asthma. The aim of this study was to measure the concentration of Alt a 1, a major allergen of A. alternata, in indoor dust samples collected in poultry farms and a sawmill using a monoclonal antibody-based enzyme immunoassay. A total of 45 dust samples were collected in poultry farms (30) and the sawmill (15) in Zagreb County (Croatia). The Alt a 1 allergen was detected in all dust samples (100%) collected in three poultry farms. The levels of Alt a 1 were in the range of 0.1-14 microg/g, and the median value was 0.37 microg/g. About 86% of dust samples contained Alt a 1 in the range of 0.1-1.0 microg/g. In the sawmill, no detectable level of Alt a 1 was found (limit of detection =0.12 microg/g). This study has shown that occupational exposure to Alt a 1 allergen in poultry farms deserves monitoring.
农场和锯木厂工人接触高浓度的过敏原真菌，如链格孢属Alternaria alternata，这与呼吸道疾病和哮喘有关。本研究的目的是使用基于单克隆抗体的酶免疫分析法测量室内灰尘样本中 Alt a 1（链格孢属 Alternaria alternata 的主要过敏原）的浓度。共采集了 45 个灰尘样本，分别来自克罗地亚萨格勒布县的 30 个家禽养殖场和 15 个锯木厂。在三个家禽养殖场采集的所有灰尘样本（100%）中都检测到了 Alt a 1 过敏原。Alt a 1 的浓度范围为 0.1-14 微克/克，中位数为 0.37 微克/克。约 86%的灰尘样本中含有 0.1-1.0 微克/克的 Alt a 1。在锯木厂中，未发现可检测到的 Alt a 1 水平（检测限=0.12 微克/克）。本研究表明，家禽养殖场中职业性接触 Alt a 1 过敏原值得监测。
