• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

A double monoclonal antibody assay for the Alternaria allergen GP70.

作者信息

Portnoy J, Pacheco F, Upadrashta B, Barnes C

机构信息

Section of Allergy/Immunology, Children's Mercy Hospital, Kansas City, Missouri.

出版信息

Ann Allergy. 1993 Oct;71(4):401-7.

PMID:7692772
Abstract

A double monoclonal antibody-based assay for the 70-kD Alternaria allergen GP70 is described. The assay is sensitive to GP70 concentrations as low as 0.2 microgram/mL and is highly specific for this allergen. The glycoprotein detected by this assay is shown to bind to human IgE from Alternaria-sensitive patients, proving that it is an allergen. Two of three commercial Alternaria preparations tested contained no detectable GP70. Several related fungi were shown to contain detectable concentrations of GP70, but the most abundant source was a commercial preparation of house dust. Further measurement of GP70 in biologic materials should increase our knowledge of the distribution and importance of this Alternaria allergen in the environment and in the development of allergic diseases.

摘要

相似文献

1
A double monoclonal antibody assay for the Alternaria allergen GP70.
Ann Allergy. 1993 Oct;71(4):401-7.
2
A sensitive two-site enzyme-linked immunosorbent assay for measurement of the major Alternaria alternata allergen Alt a 1.一种用于检测链格孢主要变应原Alt a 1的灵敏双位点酶联免疫吸附测定法。
Ann Allergy Asthma Immunol. 2003 May;90(5):529-35. doi: 10.1016/S1081-1206(10)61846-7.
3
Characterization of monoclonal antibodies to an antigenic protein from Stachybotrys chartarum and its measurement in house dust.对来自链格孢菌的一种抗原蛋白的单克隆抗体的表征及其在室内灰尘中的测定。
J Immunol Methods. 2008 Mar 20;332(1-2):121-8. doi: 10.1016/j.jim.2007.12.021. Epub 2008 Jan 29.
4
Detection of recombinant Alt a1 in a two-site, IgM based, sandwich ELISA opens up possibilities of developing alternative assays for the allergen.在基于IgM的双位点夹心ELISA中检测重组变应原Alt a1，为开发该变应原的替代检测方法开辟了可能性。
J Immunol Methods. 2006 May 30;312(1-2):111-7. doi: 10.1016/j.jim.2006.03.004. Epub 2006 Apr 21.
5
Cladosporium herbarum and Pityrosporum ovale allergen extracts share cross-reacting glycoproteins.草本枝孢菌和卵形糠秕孢子菌过敏原提取物含有交叉反应性糖蛋白。
Int Arch Allergy Immunol. 2006;140(1):30-5. doi: 10.1159/000091841. Epub 2006 Mar 3.
6
In vitro synthesis of Alternaria allergens and their recognition by murine monoclonal and human IgE antibodies.链格孢属过敏原的体外合成及其被鼠单克隆抗体和人IgE抗体识别的情况
Ann Allergy Asthma Immunol. 1997 Mar;78(3):287-92. doi: 10.1016/S1081-1206(10)63183-3.
7
Determination of Alt a 1 (Alternaria alternata) in poultry farms and a sawmill using ELISA.采用 ELISA 法测定家禽养殖场和锯木厂中的 Alt a 1（交链孢霉）。
Med Mycol. 2010 Mar;48(2):298-302. doi: 10.1080/13693780903115402.
8
IgE and monoclonal antibody binding by the mite allergen Der p 7.螨过敏原Der p 7与IgE及单克隆抗体的结合
Clin Exp Allergy. 1996 Mar;26(3):308-15.
9
A sensitive assay for the detection of IgE bound to the major birch pollen allergen, Bet v 1, in the form of immune complexes.一种用于检测以免疫复合物形式结合到主要桦树花粉过敏原Bet v 1上的IgE的灵敏检测方法。
J Immunol Methods. 2009 Jun 30;345(1-2):100-5. doi: 10.1016/j.jim.2009.04.007. Epub 2009 Apr 17.
10
Quantitation of the major fungal allergens, Alt a 1 and Asp f 1, in commercial allergenic products.商业致敏产品中主要真菌过敏原Alt a 1和Asp f 1的定量分析。
J Allergy Clin Immunol. 2001 Apr;107(4):641-6. doi: 10.1067/mai.2001.114118.

引用本文的文献

1
Evaluation of allergen vaccine potency.变应原疫苗效力评估。
Curr Allergy Asthma Rep. 2006 Sep;6(5):402-6. doi: 10.1007/s11882-996-0013-8.
2
Assessment and control of fungal allergens.真菌过敏原的评估与控制
Curr Allergy Asthma Rep. 2001 Sep;1(5):455-60. doi: 10.1007/s11882-001-0033-3.