Allergy Section, General University Hospital, Ciudad Real, Spain.
J Investig Allergol Clin Immunol. 2012;22(3):154-9.
Alternaria alternata is a risk factor for developing asthma.Alt a 1, which has been described as the major allergen in A alternata, shows a good correlation with A alternata spores only when they have germinated.
The objective of this study was to determine the correlation between spore counts and clinical symptoms in patients with allergic asthma and/or rhinitis monosensitized to A alternata.
Two types of samplers were used to determine exposure: a Burkard spore trap to collect A alternata spores and a high-volume air sampler to collect airborne particles. A total of 366 air filters were collected. Alt a 1 levels were measured by monoclonal antibody-based enzyme-linked immunosorbent assay. Eighteen monosensitized patients were asked to record their daily symptoms throughout the year.
A alternata spores were detected throughout the year, whereas Alt a 1 was detected only between March and December. Symptoms showed positive and significant correlations with spore counts (r=0.459, P<.001), and Alt a 1 levels (r=0.294, P<.001). The correlation between spores and Alt a 1 was low. The negative binomial model proved that an increase of 10 pg/m3 in Alt a 1 levels increased the number of symptoms at a 3-day lag by 5%.
In patients who are allergic to A alternata, Alt a 1 levels can be considered an important marker for predicting the risk of respiratory symptoms.
链格孢Alternaria alternata 是引发哮喘的一个风险因素。链格孢过敏原 1（Alt a 1）已被描述为链格孢菌的主要过敏原，只有当它发芽时，才与链格孢菌孢子显示出良好的相关性。
本研究的目的是确定对链格孢菌呈单一过敏的过敏性哮喘和/或鼻炎患者的孢子计数与临床症状之间的相关性。
使用两种采样器来确定暴露情况：Burkard 孢子陷阱收集链格孢菌孢子，大容量空气采样器收集空气颗粒。共采集了 366 个空气过滤器。通过基于单克隆抗体的酶联免疫吸附测定法测量 Alt a 1 水平。要求 18 名单敏患者全年记录他们的日常症状。
全年都检测到链格孢菌孢子，而 Alt a 1 仅在 3 月至 12 月之间检测到。症状与孢子计数（r=0.459，P<.001）和 Alt a 1 水平（r=0.294，P<.001）呈正显著相关。孢子和 Alt a 1 之间的相关性较低。负二项式模型证明，Alt a 1 水平增加 10 pg/m3，会使 3 天滞后的症状数量增加 5%。
在对链格孢菌过敏的患者中，Alt a 1 水平可被视为预测呼吸道症状风险的重要标志物。
