Umene Kenichi, Kawana Takashi, Fukumaki Yasuyuki
Faculty of Human Environmental Science, Department of Nutrition & Health Science, Fukuoka Woman's University, Fukuoka, Japan.
J Med Virol. 2009 Sep;81(9):1605-12. doi: 10.1002/jmv.21581.
Herpes simplex virus type 1 (HSV-1) has been reported increasingly as a cause of genital herpes, although HSV-1 is usually associated with oro-labial herpes. In the present study, serum specimens and materials for viral isolation were obtained serially from two patients with recrudescent HSV-1 genital infections to study serology and molecular epidemiology. Recurrent episodes, during which HSV-1 was isolated, were followed by an increase in the level of anti-HSV-1 antibody, suggesting a booster effect from re-exposure to viral antigens and the possible usefulness of the variation in the level of anti-HSV-1 antibody to diagnose recurrence. While genotypes of HSV-1 isolates obtained from one patient were different from those from the other patient, genotypes of sequential HSV-1 isolates obtained from the same patient were the same, implying that the recrudescent genital lesions of the two patients could be attributed to endogenous recurrence of a latent virus. Sera from one patient neutralized HSV-1 isolates obtained from the other patient as well as HSV-1 isolates obtained from the same patient. An HSV-1 isolate obtained during a later episode in one patient was neutralized by sera taken before/during the later episode of the same patient, as effectively as an HSV-1 isolate obtained during an earlier episode in the same patient; thus, in these two cases, HSV-1 was assumed to have multiplied during recurrence despite the presence of an anti-HSV-1 antibody that could neutralize experimentally HSV-1.
1型单纯疱疹病毒（HSV-1）作为生殖器疱疹的病因报告日益增多，尽管HSV-1通常与口唇疱疹相关。在本研究中，从两名复发性HSV-1生殖器感染患者中连续获取血清标本和病毒分离材料，以研究血清学和分子流行病学。在复发期分离出HSV-1，随后抗HSV-1抗体水平升高，提示再次接触病毒抗原产生了增强效应，且抗HSV-1抗体水平变化可能有助于诊断复发。虽然从一名患者分离出的HSV-1基因型与另一名患者不同，但从同一患者连续分离出的HSV-1基因型相同，这意味着两名患者的复发性生殖器损害可能归因于潜伏病毒的内源性复发。一名患者的血清可中和从另一名患者分离出的HSV-1毒株以及从同一患者分离出的HSV-1毒株。在一名患者较晚发作期分离出的HSV-1毒株，可被该患者在较晚发作期之前/期间采集的血清中和，其效果与该患者较早发作期分离出的HSV-1毒株相同；因此，在这两例中，尽管存在可在实验中中和HSV-1的抗HSV-1抗体，但仍假定HSV-1在复发期间增殖。
