Zhao Cai-xia, Liu Jia, Zhang Wei
Department of Histology, Embryology and Neurobiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 May;40(3):412-4.
To determine the expression of ciliary neurotrophic factor (CNTF) mRNA in transactional injured spinal cords and the role of CNTF in repairing and regenerating spinal cords in adult rats.
Forty clean adult Sprague Dawley (SD) rats were randomly divided into sham group and operation group (SCI group). The SCI group was subdivided into four groups, with the rats sacrificed 1, 3, 7 and 14 days after the operations (day of postoperation, DPO), respectively. The spinal cords of the rats were completely transected at T10. The rats in the sham group underwent the same procedures as those in the SCI groups except for the transection. The Basso, Beattie and Bresnahan locomotor testing scales (BBB scores) was used to evaluate the locomotor function of hindlimbs of the rats. The expression of CNTF mRNA in the spinal cords were detected with RT-PCR technique.
The BBB scores increased gradually with the time after the spinal cord injury. But the hindlimb locomotor function did not improve significantly. The CNTF mRNA in the distal stumps of injured spinal cords increased significantly on DPO 1 and 3 compared with those in the sham group (P<0.05), and then decreased from DPO 7 and returned to normal level on DPO 14.
Increased ciliary neurotrophic factor expression at the early stage after SCI may be associated with the repair and regeneration of the nerves in the rats.
确定睫状神经营养因子（CNTF）mRNA在横断性损伤脊髓中的表达以及CNTF在成年大鼠脊髓修复和再生中的作用。
40只清洁级成年Sprague Dawley（SD）大鼠随机分为假手术组和手术组（脊髓损伤组）。脊髓损伤组再分为四组，分别在术后1、3、7和14天（术后日，DPO）处死大鼠。在T10水平将大鼠脊髓完全横断。假手术组大鼠除不进行横断外，接受与脊髓损伤组相同的操作。采用Basso、Beattie和Bresnahan运动测试量表（BBB评分）评估大鼠后肢运动功能。用RT-PCR技术检测脊髓中CNTF mRNA的表达。
脊髓损伤后，BBB评分随时间逐渐增加。但后肢运动功能改善不明显。与假手术组相比，损伤脊髓远端残端的CNTF mRNA在术后1天和3天显著增加（P<0.05），然后从术后7天开始下降，在术后14天恢复到正常水平。
脊髓损伤后早期睫状神经营养因子表达增加可能与大鼠神经的修复和再生有关。
