冷冻保存样本用于纤毛活动药理学研究的适用性。

Suitability of cryopreserved samples for pharmacological studies of ciliary activity.

作者信息

Di Benedetto G, Gill J, Lopez-Vidriero M T, Clarke S W

机构信息

Department of Thoracic Medicine, Royal Free Hospital, School of Medicine, Hampstead, London, United Kingdom.

出版信息

Cryobiology. 1990 Dec;27(6):591-5. doi: 10.1016/s0011-2240(05)80026-4.

DOI:10.1016/s0011-2240(05)80026-4

PMID:1962747

Abstract

The effect of terbutaline sulfate on the ciliary activity of fresh and cryopreserved human nasal epithelium was evaluated. Cryopreservation had no effect on baseline ciliary beat frequency. Both fresh and cryopreserved samples exposed to 10(-4) M terbutaline showed a statistically significant increase in ciliary beat frequency (27 and 25%, respectively). When the percentage changes after drug challenge for fresh and cryopreserved samples were compared no statistical difference emerged. It is concluded that cryopreservation in liquid nitrogen at -196 degrees C does not affect membrane receptors, at least beta-adrenergic receptors, and therefore cryopreserved samples are suitable for pharmacological studies of ciliary activity.

摘要

评估了硫酸特布他林对新鲜和冷冻保存的人鼻上皮纤毛活性的影响。冷冻保存对基线纤毛摆动频率没有影响。暴露于10(-4)M特布他林的新鲜和冷冻保存样本的纤毛摆动频率均有统计学意义的增加（分别为27%和25%）。比较新鲜和冷冻保存样本药物激发后的百分比变化时，未出现统计学差异。得出结论，在-196℃液氮中冷冻保存不会影响膜受体，至少β-肾上腺素能受体，因此冷冻保存的样本适用于纤毛活性的药理学研究。