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范斯莱克-卡伦不可逆机制在酶促反应进程曲线分析中的应用。

Application of the Van Slyke-Cullen irreversible mechanism in the analysis of enzymatic progress curves.

作者信息

Kuzmic Petr

机构信息

BioKin Ltd., 15 Main Street Suite 232, Watertown, MA 02472, USA.

出版信息

Anal Biochem. 2009 Nov 15;394(2):287-9. doi: 10.1016/j.ab.2009.06.040. Epub 2009 Jul 21.

DOI:10.1016/j.ab.2009.06.040
PMID:19627979
Abstract

For enzymatic progress curves conforming to the Michaelis-Menten mechanism E+Sright harpoon over left harpoonES-->E+P, the minimal fitting model cast as a system of numerically integrated differential equations is the simplified, irreversible Van Slyke-Cullen mechanism E+S-->ES-->E+P. The best-fit value of the bimolecular association rate constant is identical to the specificity constant kcat/KM. An illustrative example involves a fluorogenic continuous assay of the HIV protease, analyzed by the differential-equation oriented software package DYNAFIT [P. Kuzmic, Anal. Biochem. 237 (1996) 260].

摘要

对于符合米氏机制E+S⇌ES→E+P的酶促进程曲线，作为数值积分微分方程组的最小拟合模型是简化的不可逆范斯莱克 - 卡伦机制E+S→ES→E+P。双分子缔合速率常数的最佳拟合值与特异性常数kcat/KM相同。一个示例涉及通过面向微分方程的软件包DYNAFIT [P. 库兹米克，《分析生物化学》237 (1996) 260] 分析的HIV蛋白酶的荧光连续测定。

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