Kuzmic Petr
BioKin Ltd., 1652 South Grand Avenue, Suite 337, Pullman, WA 99163, USA.
Mol Cell Endocrinol. 2006 Mar 27;248(1-2):172-81. doi: 10.1016/j.mce.2005.10.012. Epub 2005 Dec 20.
A generalized numerical treatment of rapid-equilibrium enzyme kinetics is presented. This new approach relies on automatic computer derivation of the underlying mathematical model (a system of simultaneous nonlinear algebraic equations) from a symbolic representation of the reaction mechanism (a system of biochemical equations) provided by the researcher. The method allows experimental biochemists to analyze initial-rate enzyme kinetic data without having to use any mathematical equations. An illustrative example is based on the inhibition kinetics of 17beta-hydroxysteroid dehydrogenase type 5 by a class of natural compounds. A computer implementation of the new method, a newly modified software package DYNAFIT [Kuzmic, P., 1996. Program DYNAFIT for the analysis of enzyme kinetic data: application to HIV proteinase. Anal. Biochem. 237, 260-273], is freely available to all academic researchers.
本文提出了一种快速平衡酶动力学的广义数值处理方法。这种新方法依赖于根据研究人员提供的反应机制（生化方程系统）的符号表示，自动由计算机推导基础数学模型（一个联立非线性代数方程组）。该方法使实验生物化学家能够分析酶促反应初速率动力学数据，而无需使用任何数学方程。一个说明性的例子基于一类天然化合物对5型17β-羟基类固醇脱氢酶的抑制动力学。新方法的计算机实现程序，即一个新修改的软件包DYNAFIT [库兹米克，P.，1996年。用于分析酶动力学数据的程序DYNAFIT：应用于HIV蛋白酶。分析生物化学。237，260 - 273]，可供所有学术研究人员免费使用。
