Martins-Oliveira Alisson, Speciali Jose G, Dach Fabiola, Marcaccini Andrea M, Gonçalves Flavia M, Gerlach Raquel F, Tanus-Santos J E
Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirao Preto, SP, Brazil.
Clin Chim Acta. 2009 Oct;408(1-2):60-4. doi: 10.1016/j.cca.2009.07.008. Epub 2009 Jul 20.
We compared the circulating levels of matrix metalloproteinase (MMP)-2, MMP-9, tissue inhibitors of metalloproteinase (TIMP)-1, TIMP-2, and MMP-9/TIMP-1 and MMP-2/TIMP-2 ratios in migraine patients without aura (MWA) and in migraine patients with aura (MA) with those found in healthy subjects (controls).
We studied 80 migraine (40 MWA and 40 MA) women and 40 controls. Pro-MMP-2 levels were determined by zymography and MMP-9, TIMP-1, and TIMP-2 levels were determined by ELISA.
While we found similar TIMP-2 levels, higher plasma pro-MMP-2 and pro-MMP-2/TIMP-2 ratios were found in MWA and MA patients compared with controls (P<0.05). Higher TIMP-1 levels and lower MMP-9/TIMP-1 ratios were found in MA, but not in MWA, patients compared with controls (P<0.05). We found no significant differences when patients without headache attack were compared with patients having a headache attack (all P<0.05).
We showed an increased net MMP-2 activity in MWA and MA. The increased MMP-9/TIMP-1 ratios in MWA patients contrast with the lower MMP-9/TIMP-1 ratios in MA patients and may reflect pathophysiological differences between these conditions.
我们比较了无先兆偏头痛（MWA）患者、有先兆偏头痛（MA）患者与健康受试者（对照组）体内基质金属蛋白酶（MMP）-2、MMP-9、金属蛋白酶组织抑制剂（TIMP）-1、TIMP-2的循环水平，以及MMP-9/TIMP-1和MMP-2/TIMP-2的比值。
我们研究了80名偏头痛女性（40名MWA患者和40名MA患者）和40名对照者。采用酶谱法测定前MMP-2水平，采用酶联免疫吸附测定法测定MMP-9、TIMP-1和TIMP-2水平。
虽然我们发现TIMP-2水平相似，但与对照组相比，MWA和MA患者的血浆前MMP-2水平及前MMP-2/TIMP-2比值更高（P<0.05）。与对照组相比，MA患者而非MWA患者的TIMP-1水平更高，MMP-9/TIMP-1比值更低（P<0.05）。将无头痛发作的患者与有头痛发作的患者进行比较时，我们未发现显著差异（所有P<0.05）。
我们发现MWA和MA患者的MMP-2净活性增加。MWA患者中升高的MMP-9/TIMP-1比值与MA患者中降低的MMP-9/TIMP-1比值形成对比，这可能反映了这些情况之间的病理生理差异。
