Takahashi S, Kazuyama Y
Department of Dermatology of Keio University School of Medicine.
Kansenshogaku Zasshi. 1990 Nov;64(11):1367-71. doi: 10.11150/kansenshogakuzasshi1970.64.1367.
We performed the in situ hybridization (ISH) study of the smear cells taken from the patients of herpes zoster. We used the biotinylated EcoRI-B, H fragment of varicella-zoster-virus (VZV) DNA as the probe. In the control study of ISH VZV-infected cells showed strong staining, but no staining was observed in the infected cells by other herpes virus. We examined the smear cells of 19 patients of herpes zoster and 2 patients of herpes simplex by ISH. 30 of the 35 specimens of herpes zoster was positive by ISH. We detected VZV DNA in the smear cells of all cases of the bullous stage, but the sensitivity was about 75% in the later stage. Smear cells of herpes simplex was not stained by ISH. We also performed virus isolation of 13 cases of herpes zoster. But the sensitivity of ISH was higher than that of virus isolation in the other stages of herpes zoster. This method is very simple and can be completed in about 1 hour. It also has high specificity and sensitivity. So this method is very useful for the rapid diagnosis of VZV infection.
我们对带状疱疹患者的涂片细胞进行了原位杂交（ISH）研究。我们使用生物素化的水痘 - 带状疱疹病毒（VZV）DNA的EcoRI - B、H片段作为探针。在ISH的对照研究中，VZV感染的细胞显示出强染色，但其他疱疹病毒感染的细胞未观察到染色。我们通过ISH检查了19例带状疱疹患者和2例单纯疱疹患者的涂片细胞。35份带状疱疹标本中有30份ISH呈阳性。我们在所有大疱期病例的涂片细胞中检测到了VZV DNA，但在后期敏感性约为75%。单纯疱疹的涂片细胞未被ISH染色。我们还对13例带状疱疹病例进行了病毒分离。但在带状疱疹的其他阶段，ISH的敏感性高于病毒分离。该方法非常简单，大约1小时即可完成。它还具有高特异性和敏感性。因此，该方法对VZV感染的快速诊断非常有用。
