Pinheiro Céline, Longatto-Filho Adhemar, Simões Kleber, Jacob Carlos Eduardo, Bresciani Cláudio José Caldas, Zilberstein Bruno, Cecconello Ivan, Alves Venâncio Avancini Ferreira, Schmitt Fernando, Baltazar Fátima
Life and Health Sciences Research Institute, School of Health Sciences, University of Minho, 4710-057 Braga, Portugal.
Eur J Cancer. 2009 Sep;45(13):2418-24. doi: 10.1016/j.ejca.2009.06.018. Epub 2009 Jul 21.
The aim of the present work was to assess the role of monocarboxylate transporters (MCTs), namely MCT1 and MCT4 as well as MCT/CD147 co-expression in gastric tissues and evaluate their clinico-pathological significance in gastric carcinoma. For that, we analysed the immunohistochemical expression of MCT1, MCT4 and CD147, in a large series of gastric samples, including non-neoplastic, tumour and metastatic tissues. A significant decrease in MCT4 plasma membrane expression was observed from non-neoplastic to gastric primary malignant tissues and to lymph-node metastasis and both MCT1 and MCT4 correlated with CD147. Importantly, both MCT4 and CD147 were more frequently expressed in Lauren's intestinal-type tumours and MCT1/CD147 co-expression was associated with advanced gastric carcinoma, Lauren's intestinal type, TNM staging and lymph-node metastasis. Our results showed that the prognostic value of CD147 was associated with MCT1 co-expression in gastric cancer cells, supporting the view that CD147 plasma membrane activity is dependent on MCT co-expression.
本研究的目的是评估单羧酸转运蛋白（MCTs），即MCT1和MCT4以及MCT/CD147共表达在胃组织中的作用，并评估它们在胃癌中的临床病理意义。为此，我们分析了一系列胃样本（包括非肿瘤、肿瘤和转移组织）中MCT1、MCT4和CD147的免疫组化表达。从非肿瘤组织到胃原发性恶性组织再到淋巴结转移，观察到MCT4质膜表达显著降低，且MCT1和MCT4均与CD147相关。重要的是，MCT4和CD147在劳伦肠型肿瘤中更频繁表达，且MCT1/CD147共表达与进展期胃癌、劳伦肠型、TNM分期和淋巴结转移相关。我们的结果表明，CD147的预后价值与胃癌细胞中MCT1共表达相关，支持CD147质膜活性依赖于MCT共表达的观点。
