Zhao Xin-Qing, Li Wen-Jun, Jiao Wen-Ce, Li Yan, Yuan Wen-Jie, Zhang Yu-Qin, Klenk Hans-Peter, Suh Joo-Won, Bai Feng-Wu
Department of Bioscience and Bioengineering, Dalian University of Technology, Dalian 116024, People's Republic of China.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2870-4. doi: 10.1099/ijs.0.009878-0. Epub 2009 Jul 23.
A novel actinomycete, strain S187(T), was isolated from a marine sediment sample collected from Xinghai Bay, Dalian, China. Growth occurred on ISP medium 2 containing 0-9 % NaCl and at pH 6.0-9.0 and 10-45 degrees C. The cell wall of strain S187(T) contained the isomer ll-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H(6)) (40.8 %), MK-9(H(8)) (38.2 %) and MK-9(H(2)) (8.8 %). The major fatty acids were iso-C(16 : 0) (29.6 %), anteiso-C(15 : 0) (14.0 %) and anteiso-C(17 : 0) (11.6 %). Cells contained phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannosides and one unknown phospholipid. The G+C content of the genomic DNA was 72.01 mol%. The 16S rRNA gene sequence of the isolate had similarities of 98.1 and 97.5 % with those of Streptomyces flavofuscus NRRL B-8036(T) (=DSM 41426(T)) and Streptomyces albiaxialis DSM 41799(T), respectively, showing that the novel strain should be assigned to the genus Streptomyces. DNA-DNA hybridizations with the two above-mentioned Streptomyces species showed 31.4 and 46.9 % relatedness, respectively. Moreover, the three strains differed in some physiological and biochemical properties. Thus, on the basis of phenotypic and genotypic analyses, it is proposed that strain S187(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces xinghaiensis sp. nov. is proposed; the type strain is S187(T) (=NRRL B-24674(T)=CCTCC AA 208049(T)=KCTC 19546(T)).
从中国大连星海湾采集的海洋沉积物样品中分离出一株新型放线菌，菌株S187(T)。该菌株在含0 - 9%氯化钠、pH值为6.0 - 9.0且温度为10 - 45℃的ISP2培养基上生长。菌株S187(T)的细胞壁含有异构型ll - 二氨基庚二酸作为特征性二氨基酸。主要的甲基萘醌为MK - 9(H(6))（40.8%）、MK - 9(H(8))（38.2%）和MK - 9(H(2))（8.8%）。主要脂肪酸为异-C(16 : 0)（29.6%）、anteiso-C(15 : 0)（14.0%）和anteiso-C(17 : 0)（11.6%）。细胞含有磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰甘油、磷脂酰肌醇甘露糖苷和一种未知磷脂。基因组DNA的G + C含量为72.01 mol%。该分离株的16S rRNA基因序列与黄褐链霉菌NRRL B - 8036(T)（=DSM 41426(T)）和白轴链霉菌DSM 41799(T)的相似性分别为98.1%和97.5%，表明该新菌株应归属于链霉菌属。与上述两种链霉菌进行的DNA - DNA杂交显示相关性分别为31.4%和46.9%。此外，这三株菌在一些生理生化特性上存在差异。因此，基于表型和基因型分析，建议菌株S187(T)代表链霉菌属的一个新物种，命名为星海链霉菌（Streptomyces xinghaiensis sp. nov.）；模式菌株为S187(T)（=NRRL B - 24674(T)=CCTCC AA 208049(T)=KCTC 19546(T)）。
