Wang Yun, Cao Lan-Lan, Tang Shu-Kun, Lou Kai, Mao Pei-Hong, Jin Xiang, Jiang Cheng-Lin, Xu Li-Hua, Li Wen-Jun
Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang 830091, People's Republic of China.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2875-9. doi: 10.1099/ijs.0.009670-0. Epub 2009 Jul 23.
A Gram-positive, aerobic, motile, coccoid, orange-pigmented bacterium, designated strain YIM 91094(T), was isolated from a salt lake sample collected from Barkol Lake in Xinjiang Province, north-west China. The strain was able to grow at pH 6.0-8.0 (optimal growth at pH 7.0), at 10-37 degrees C (optimal growth at 28 degrees C) and in the presence of 0-25 % (w/v) NaCl [optimal growth in the presence of 10-15 % (w/v) NaCl]. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain YIM 91094(T) was affiliated with the genus Marinococcus and exhibited levels of sequence similarity of 99.2 % to Marinococcus halotolerans YIM 70157(T) and 99.7 % to Marinococcus halophilus DSM 20408(T). However, it showed moderately low levels of DNA-DNA relatedness with the above type strains (56.0 and 57.5 %, respectively). The peptidoglycan type of strain YIM 91094(T) was A1gamma, with meso-diaminopimelic acid as the diagnostic diamino acid. MK-7 was the predominant menaquinone and anteiso-C(15 : 0) (49.9 % of the total) and anteiso-C(17 : 0) (29.6 %) were the major cellular fatty acids. The DNA G+C content was 48.7 mol%. Strain YIM 91094(T) possessed chemotaxonomic markers that were consistent with its classification in the genus Marinococcus. On the basis of the data presented, strain YIM 91094(T) is considered to represent a novel species of the genus Marinococcus, for which the name Marinococcus luteus sp. nov. is proposed. The type strain is YIM 91094(T) (=KCTC 13214(T)=CCTCC AA 208014(T)). An emended description of the genus Marinococcus is provided.
从中国西北部新疆维吾尔自治区巴里坤湖采集的盐湖样品中分离出一株革兰氏阳性、需氧、运动、球状、产橙色色素的细菌，命名为菌株YIM 91094(T)。该菌株能够在pH 6.0 - 8.0（最适生长pH 7.0）、10 - 37℃（最适生长温度28℃）以及0 - 25%（w/v）NaCl存在的条件下生长[在10 - 15%（w/v）NaCl存在时生长最佳]。基于16S rRNA基因序列的系统发育分析表明，菌株YIM 91094(T)隶属于海球菌属，与耐盐海球菌YIM 70157(T)的序列相似性为99.2%，与嗜盐海球菌DSM 20408(T)的序列相似性为99.7%。然而，它与上述模式菌株的DNA - DNA相关性水平较低（分别为56.0%和57.5%）。菌株YIM 91094(T)的肽聚糖类型为A1γ型，以中 - 二氨基庚二酸作为诊断性二氨基酸。MK - 7是主要的甲基萘醌，anteiso - C(15 : 0)（占总量的49.9%）和anteiso - C(17 : 0)（29.6%）是主要的细胞脂肪酸。DNA的G + C含量为48.7 mol%。菌株YIM 91094(T)具有与海球菌属分类一致的化学分类学特征。根据所提供的数据，菌株YIM 91094(T)被认为代表海球菌属的一个新物种，为此提出新名称黄色海球菌（Marinococcus luteus sp. nov.）。模式菌株为YIM 91094(T)（=KCTC 13214(T)=CCTCC AA 208014(T)）。本文还提供了海球菌属的修订描述。
