Piaggio Giovanna, Rosti Vittorio, Corselli Mirko, Bertolotti Francesca, Bergamaschi Gaetano, Pozzi Sarah, Imperiale Davide, Chiavarina Barbara, Bonetti Elisa, Novara Francesca, Sessarego Mario, Villani Laura, Garuti Anna, Massa Margherita, Ghio Riccardo, Campanelli Rita, Bacigalupo Andrea, Pecci Alessandro, Viarengo Gianluca, Zuffardi Orsetta, Frassoni Francesco, Barosi Giovanni
Divisione Ematologia, Ospedale San Martino, Genova.
Blood. 2009 Oct 1;114(14):3127-30. doi: 10.1182/blood-2008-12-190991. Epub 2009 Jul 23.
Two putative types of circulating endothelial progenitor cells have been recently identified in vitro: (1) endothelial colony-forming cell (ECFC) and (2) colony-forming unit-endothelial cell (CFU-EC). Only the former is now recognized to belong to endothelial lineage. We have used the ECFC and CFU-EC assays to readdress the issue of the clonal relation between endothelial progenitor cells and hematopoietic stem cells in patients with Philadelphia-positive and Philadelphia-negative chronic myeloproliferative disorders. Both ECFCs and CFU-ECs were cultured from peripheral blood mononuclear cells, and either BCR-ABL rearrangement or JAK2-V617F mutation were assessed in both types of endothelial colonies. We found that ECFCs lack the disease-specific markers, which are otherwise present in CFU-ECs, thus reinforcing the concept that the latter belongs to the hematopoietic lineage, and showing that in chronic myeloproliferative disorders the cell that gives rise to circulating ECFC has a distinct origin from the cell of the hematopoietic malignant clone.
（1）内皮集落形成细胞（ECFC）和（2）内皮细胞集落形成单位（CFU-EC）。目前仅前者被认为属于内皮谱系。我们使用ECFC和CFU-EC检测方法，重新探讨费城染色体阳性和费城染色体阴性慢性骨髓增殖性疾病患者中内皮祖细胞与造血干细胞之间的克隆关系问题。从外周血单个核细胞中培养出ECFC和CFU-EC，并在这两种类型的内皮集落中评估BCR-ABL重排或JAK2-V617F突变。我们发现ECFC缺乏疾病特异性标志物，而这些标志物在CFU-EC中存在，从而强化了后者属于造血谱系的概念，并表明在慢性骨髓增殖性疾病中，产生循环ECFC的细胞与造血恶性克隆细胞具有不同的起源。
