Artyukova Elena V, Kozyrenko Marina M, Gorovoy Peter G, Zhuravlev Yury N
Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, 690022 Vladivostok, Russia.
Genetica. 2009 Nov;137(2):201-12. doi: 10.1007/s10709-009-9386-7. Epub 2009 Jul 24.
Microbiota decussata Kom. (Cupressaceae) is a subalpine species endemic to the Sikhote Alin Mountains with populations scattered throughout the range. We used sequence data for four noncoding regions of chloroplast DNA to characterize the genetic diversity in populations sampled from different parts of M. decussata natural range. No variation was observed in the trnT-trnF region, whereas the trnH-psbA, trnS-trnfM, and trnS-trnG regions showed polymorphisms. At the species level, we found a low nucleotide diversity (pi = 0.0009) and high haplotype diversity (h = 0.981) as well as high differentiation (Phi(ST) = 0.420). N(ST) and G(ST) values suggested the existence of a phylogeographic structure in M. decussata. The observed patterns of diversity could be explained in part by ecological features of the species and its long-term persistence throughout the range with population expansion, successive fragmentation and isolation.
交让木（Microbiota decussata Kom.，柏科）是锡霍特山脉特有的亚高山物种，其种群分布在整个山脉范围内。我们利用叶绿体DNA四个非编码区的序列数据，对从交让木自然分布范围不同区域采集的种群的遗传多样性进行了表征。在trnT-trnF区域未观察到变异，而trnH-psbA、trnS-trnfM和trnS-trnG区域表现出多态性。在物种水平上，我们发现核苷酸多样性较低（π = 0.0009），单倍型多样性较高（h = 0.981），且分化程度较高（Phi(ST) = 0.420）。N(ST)和G(ST)值表明交让木存在系统发育地理结构。观察到的多样性模式部分可以由该物种的生态特征及其在整个分布范围内长期存在、种群扩张、连续片段化和隔离来解释。
