Nur I, Elkaim R, Herzberg M
Orgenics Ltd, Yavne, Israel.
Ann Biol Clin (Paris). 1990;48(8):557-9.
A technique is described in which unlabeled DNA probes are immobilized on the plastic surface of a 12-tooth comb and used to capture homologs by a hybridization procedure. In the examples described, cellular DNA from cervical biopsies is chemically labeled using the Chemi-probe system. The sample containing labeled DNA is then hybridized with the immobilized unlabeled probe (reverse hybridization). By means of this technique, human papillomavirus sequences can be detected with a sensitivity comparable to that of radioactive probe procedures. DNA hybrids are visualized as colored spots on each tooth by moving the comb through the reagent solutions of the prefilled developing plate. The whole procedure requires less than 50 minutes hands-on time, and the results are obtained in a few hours. Application of the technique to the detection and typing of mycoplasma DNA is also reported.
本文描述了一种技术，其中未标记的DNA探针固定在12齿梳的塑料表面，并通过杂交程序用于捕获同源物。在所述示例中，使用化学探针系统对宫颈活检的细胞DNA进行化学标记。然后将含有标记DNA的样品与固定的未标记探针杂交（反向杂交）。通过这种技术，可以检测人乳头瘤病毒序列，其灵敏度与放射性探针程序相当。通过将梳子移动通过预填充显影板的试剂溶液，DNA杂交体在每个齿上可视化为彩色斑点。整个过程实际操作时间不到50分钟，结果在几小时内即可获得。还报道了该技术在支原体DNA检测和分型中的应用。
