Skriabin G A, Petruniaka V V, Orlov S N, Kotelevtsev S V, Kozlov Iu P
Biokhimiia. 1990 Aug;55(8):1503-6.
The activities of Ca-ATPase and Na,K-ATPase in saponin-treated erythrocytes of man, rat and carp were compared. It was shown that at free calcium concentrations lower than 1 microM the activity of Ca-ATPase in carp erythrocytes was by one order of magnitude lower than in rat erythrocytes and 3-4 times lower than in human red blood cells. At [Ca2+] = 0.4 microM the activities of Na,K-ATPase in all species under study were essentially the same. The increase in Ca2+ concentration up to 1 microM resulted in a 2-5-fold activation of Na,K-ATPase in rat and carp erythrocytes, respectively. In all cases studied a further elevation of free calcium concentration was accompanied by a decline of the Na,K-ATPase activity. It was shown that the Pi content in carp erythrocytes is 5-6 times as high as that in mammalian cells. This circumstance is a considerable obstacle to a detailed analysis of mechanisms of ATPase activity regulation in carp erythrocytes by methods used for determination of inorganic phosphate production.
比较了皂角苷处理的人、大鼠和鲤鱼红细胞中钙 - ATP酶和钠钾 - ATP酶的活性。结果表明，在游离钙浓度低于1微摩尔时，鲤鱼红细胞中钙 - ATP酶的活性比大鼠红细胞低一个数量级，比人类红细胞低3 - 4倍。在[Ca2+]=0.4微摩尔时，所有研究物种中钠钾 - ATP酶的活性基本相同。钙浓度增加到1微摩尔分别导致大鼠和鲤鱼红细胞中钠钾 - ATP酶活性激活2 - 5倍。在所有研究的情况下，游离钙浓度进一步升高伴随着钠钾 - ATP酶活性下降。结果表明，鲤鱼红细胞中无机磷酸含量比哺乳动物细胞高5 - 6倍。这种情况极大地阻碍了用测定无机磷酸盐生成的方法详细分析鲤鱼红细胞中ATP酶活性调节机制。
