Kazennov A M, Maslova M N, Savina G V
Zh Evol Biokhim Fiziol. 1984 Mar-Apr;20(2):167-73.
Studies have been made on some of the properties of Na,K-ATPase of a nuclear erythrocytes of man and nuclear erythrocytes of the carp Cyprinus carpio. Human erythrocytes yielded the enzymic activity only after their treatment by a detergent Twin-20; under optimal conditions, it amounted to 2.6 mcmole /ml of erythrocytes per 1 h. In carp erythrocytes, Na,K-ATPase activity could be detected without detergent treatment, being 10-fold higher under optimal conditions than that in human erythrocytes. Repetitive washing of carp erythrocytes from the plasma (for more than 3 times), significantly increased their viscosity and resulted in spontaneous hemolysis. Simultaneously , the activity of Na,K-ATPase increased 2-10 times depending on the composition of incubation media. Under these conditions, the pattern of changes in the enzymatic activity, resulting from shifts in Mg2+ and EDTA concentrations, was altered. The presence of latent Na,K-ATPase activity in the erythrocytes in explained by a low permeability of membranes to ATP and ions. Exogeneous ATP cannot be utilized by the enzyme in the intact human erythrocytes, whereas intact carp erythrocytes exhibit significant permeability to the exogeneous substrate. It is suggested that in vivo this fact may be of physiological importance.
对人类有核红细胞和鲤鱼（Cyprinus carpio）有核红细胞的钠钾 - ATP酶的一些特性进行了研究。人类红细胞只有在用去污剂吐温 - 20处理后才产生酶活性；在最佳条件下，每小时每毫升红细胞的酶活性为2.6微摩尔。在鲤鱼红细胞中，无需去污剂处理就能检测到钠钾 - ATP酶活性，在最佳条件下其活性比人类红细胞高10倍。从血浆中反复洗涤鲤鱼红细胞（超过3次），会显著增加其粘度并导致自发性溶血。同时，根据孵育介质的组成，钠钾 - ATP酶的活性会增加2至10倍。在这些条件下，由镁离子和乙二胺四乙酸（EDTA）浓度变化引起的酶活性变化模式发生了改变。红细胞中存在潜在的钠钾 - ATP酶活性是由于细胞膜对ATP和离子的低通透性所致。完整的人类红细胞中的酶无法利用外源ATP，而完整的鲤鱼红细胞对外源底物具有显著的通透性。有人认为，在体内这一事实可能具有生理重要性。
