Park Young Kyoung, Choi Ji Young, Jung Sook-In, Park Kyong-Hwa, Lee Hyuck, Jung Dong Sik, Heo Sang Taek, Kim Shin-Woo, Chang Hyun-Ha, Cheong Hae Suk, Chung Doo Ryeon, Peck Kyong Ran, Song Jae-Hoon, Ko Kwan Soo
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea.
Diagn Microbiol Infect Dis. 2009 Aug;64(4):389-95. doi: 10.1016/j.diagmicrobio.2009.03.029.
We investigated the characteristics of 48 carbapenem-resistant Acinetobacter baumannii isolates collected from 5 tertiary care hospitals in Korea by multilocus sequencing typing, pulsed-field gel electrophoresis, and polymerase chain reaction amplification of the antimicrobial resistance determinants. We identified 2 distinct main clones of carbapenem-resistant A. baumannii isolates, which showed different antimicrobial resistance profiles and are also differentiated by the kinds of oxacillinase (OXA) carbapenemases and Acinetobacter-derived cephalosporinase (ADC) beta-lactamases. One main clone, ST22:A, had 27 carbapenem-resistant isolates (56.3%), showed high polymyxin B and colistin resistances (33.3% and 37.0%, respectively), and contained both bla(OXA-51-like) and bla(OXA-23-like) genes and the bla(ADC-29) or bla(ADC-30) gene. In contrast, the other main clone, ST28:B, included 15 isolates (31.3%), showed complete susceptibilities to polymyxin B and colistin, and contained only the bla(OXA-51-like) gene and bla(ADC-31) or bla(ADC-32) genes. The distribution of these main carbapenem-resistant A. baumannii clones did not relate to locality, indicating that they are widespread in Korean hospitals. In addition, we found new types of PER beta-lactamases, PER-6.
我们通过多位点测序分型、脉冲场凝胶电泳以及抗菌药物耐药决定簇的聚合酶链反应扩增，对从韩国5家三级医疗医院收集的48株耐碳青霉烯鲍曼不动杆菌分离株的特征进行了研究。我们鉴定出耐碳青霉烯鲍曼不动杆菌分离株的2个不同主要克隆，它们表现出不同的抗菌药物耐药谱，并且还可通过奥沙西林酶（OXA）碳青霉烯酶和鲍曼不动杆菌衍生头孢菌素酶（ADC）β-内酰胺酶的种类加以区分。一个主要克隆ST22:A有27株耐碳青霉烯分离株（56.3%），表现出对多粘菌素B和黏菌素的高耐药性（分别为33.3%和37.0%），并且含有bla(OXA-51-like)和bla(OXA-23-like)基因以及bla(ADC-29)或bla(ADC-30)基因。相比之下，另一个主要克隆ST28:B包括15株分离株（31.3%），对多粘菌素B和黏菌素完全敏感，并且仅含有bla(OXA-51-like)基因以及bla(ADC-31)或bla(ADC-32)基因。这些主要的耐碳青霉烯鲍曼不动杆菌克隆的分布与地理位置无关，表明它们在韩国医院广泛存在。此外，我们发现了新型的PERβ-内酰胺酶，即PER-6。
