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通过实时聚合酶链反应快速鉴定肠炎沙门氏菌亚利桑那亚种和肠炎沙门氏菌亚迪亚利桑那亚种

Rapid identification of Salmonella enterica subsp. arizonae and S. enterica subsp. diarizonae by real-time polymerase chain reaction.

作者信息

Hopkins Katie L, Peters Tansy M, Lawson Andy J, Owen Robert J

机构信息

Department of Gastrointestinal, Emerging and Zoonotic Infections, Health Protection Agency Centre for Infections, NW9 5EQ London, United Kingdom.

出版信息

Diagn Microbiol Infect Dis. 2009 Aug;64(4):452-4. doi: 10.1016/j.diagmicrobio.2009.03.022.

Abstract

Reptiles are popular as pets, leading to an increased risk of human infections due to uncommon Salmonella strains including the Arizona group (subspecies arizonae and diarizonae). We present a real-time Arizona-specific polymerase chain reaction demonstrating 100% specificity and 99.6% sensitivity, offering savings in time and labor over traditional identification methods.

摘要

爬行动物作为宠物很受欢迎,这导致人类因包括亚利桑那菌群(亚利桑那亚种和双亚利桑那亚种)在内的不常见沙门氏菌菌株而感染的风险增加。我们展示了一种针对亚利桑那菌群的实时聚合酶链反应,其特异性为100%,灵敏度为99.6%,与传统鉴定方法相比,节省了时间和人力。

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