Poultry Diagnostic and Research Center, Department of Population Health, University of Georgia College of Veterinary Medicine, Athens, GA 30602.
USDA-APHIS National Veterinary Services Laboratories, Ames, IA 50010.
Avian Dis. 2020 Sep 1;64(3):305-309. doi: 10.1637/aviandiseases-D-19-00197.
Salmonella enterica subspecies arizonae (subspecies IIIa) is most frequently associated with reptiles but is also a bacterial pathogen of poultry, primarily of young turkeys where it induces septicemia, neurologic signs, and increased mortality. Arizonosis clinical cases in broiler chickens have recently been documented in the United States, driving the development of a rapid, molecular-based diagnostic for this subspecies. S. enterica subsp. arizonae is a genetically distinct subgroup of S. enterica, primarily diagnosed through culture followed by serotyping or biochemical identification, which are costly in both time and laboratory resources. Real-time/quantitative PCR offers rapid and sensitive detection of Salmonella sp. in laboratory and diagnostic samples; however, no such methodology exists to differentiate S. enterica subsp. arizonae from other Salmonella sp. In this study, we designed a quantitative PCR assay for S. enterica subsp. arizonae. The assay is able to differentiate S. enterica subsp. arizonae from other S. enterica subspecies, including S. enterica subsp. diarizonae (IIIb), and other non-Salmonella bacteria. Validation, including 56 different S. enterica subsp. arizonae serovars, demonstrated 100% sensitivity and 100% specificity. This assay provides a rapid diagnostic option for suspected cases of arizonosis in poultry.
亚利桑那沙门氏菌亚种（亚种 IIIa）最常与爬行动物有关，但也是家禽的细菌病原体，主要是幼火鸡，它会引起败血病、神经症状和死亡率增加。最近在美国记录了肉鸡中阿利桑那菌病的临床病例，这推动了针对该亚种的快速分子诊断方法的发展。亚利桑那沙门氏菌亚种是沙门氏菌的一个遗传上不同的亚群，主要通过培养后血清分型或生化鉴定来诊断，这在时间和实验室资源方面都很昂贵。实时/定量 PCR 提供了在实验室和诊断样本中快速灵敏地检测沙门氏菌的方法；然而，目前还没有这样的方法可以将亚利桑那沙门氏菌亚种与其他沙门氏菌区分开来。在这项研究中，我们设计了一种用于亚利桑那沙门氏菌亚种的定量 PCR 检测方法。该检测方法能够将亚利桑那沙门氏菌亚种与其他沙门氏菌亚种（包括 IIIb 型的沙门氏菌亚种 diarizonae 和其他非沙门氏菌）区分开来。验证包括 56 种不同的亚利桑那沙门氏菌亚种血清型，证明了 100%的敏感性和 100%的特异性。该检测方法为家禽中疑似阿利桑那菌病提供了快速诊断选择。
