血清25-羟基维生素D和1,25-二羟基维生素D定量自动化方法的比较

A comparison of automated methods for the quantitation of serum 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D.

作者信息

Kimball Samantha M, Vieth Reinhold

机构信息

Department of Nutritional Sciences, University of Toronto, Department of Laboratory Medicine and Pathology, Mt. Sinai Hospital, Ontario, Canada.

出版信息

Clin Biochem. 2007 Nov;40(16-17):1305-10. doi: 10.1016/j.clinbiochem.2007.07.015. Epub 2007 Aug 11.

DOI:10.1016/j.clinbiochem.2007.07.015

PMID:17825808

Abstract

OBJECTIVE

To compare automated platforms with the routinely used methods in our clinical laboratory for the quantitation of 25-hydroxyvitamin D [25(OH)D] and 1,25-dihydroxyvitamin D [1,25(OH)2D].

METHOD

The NEXgen Four and Triturus ELISA platforms, utilizing the IDS enzyme immunoassay (EIA) kit for 25(OH)D, and the DiaSorin Liaison 25(OH)D methods were compared with the DiaSorin radio immunoassay (RIA) kit. The NEXgen Four and the Triturus, utilizing IDS EIA for 1,25(OH)2D, were compared with the thymus radioreceptor assay (RRA) for measurement of 1,25(OH)2D.

RESULTS

NEXgen correlated best with DiaSorin RIA (r(2)=0.652). NEXgen correlated best with the thymus RRA method (r(2)=0.541). Imprecision CV values for NEXgen 1,25(OH)2D were 2.8-9.4% within-run and 10.2-13.9% between-run compared with a between-run precision of 14.0-16.9% with the thymus RRA method.

CONCLUSION

NEXgen correlated best with DiaSorin RIA for measurement of 25(OH)D. NEXgen correlated best and demonstrated better precision than thymus RRA for quantitation of 1,25(OH)2D.

摘要

目的

比较自动化检测平台与我们临床实验室常规使用的方法对25-羟基维生素D[25(OH)D]和1,25-二羟基维生素D[1,25(OH)2D]进行定量分析的结果。

方法

将采用IDS酶免疫分析法（EIA）试剂盒检测25(OH)D的NEXgen Four和Triturus酶联免疫吸附测定（ELISA）平台以及DiaSorin Liaison 25(OH)D检测方法与DiaSorin放射免疫分析法（RIA）试剂盒进行比较。将采用IDS EIA检测1,25(OH)2D的NEXgen Four和Triturus平台与用于测量1,25(OH)2D的胸腺放射受体分析法（RRA）进行比较。

结果

NEXgen与DiaSorin RIA的相关性最佳（r(2)=0.652）。NEXgen与胸腺RRA方法的相关性最佳（r(2)=0.541）。NEXgen 1,25(OH)2D的批内不精密度CV值为2.8 - 9.4%，批间不精密度CV值为10.2 - 13.9%，而胸腺RRA方法的批间精密度为14.0 - 16.9%。