Laboratory of Plant Biochemistry & Biotechnology, Department of Plant Sciences, Technological Educational Institute of Crete, P.O. Box 1939, Heraklion GR 71004, Greece.
Metab Eng. 2009 Nov;11(6):355-66. doi: 10.1016/j.ymben.2009.07.004. Epub 2009 Jul 22.
Chemical or biological synthesis of plant secondary metabolites has attracted increasing interest due to their proven or assumed beneficial properties and health promoting effects. Resveratrol, a stilbenoid, naringenin, a flavanone, genistein, an isoflavone, and the flavonols kaempferol and quercetin have been shown to possess high nutritional and agricultural value. Four metabolically engineered yeast strains harboring plasmids with heterologous genes for enzymes involved in the biosynthesis of these compounds from phenylalanine have been constructed. Time course analyses of precursor utilization and end-product accumulation were carried out establishing the production of 0.29-0.31 mg/L of trans-resveratrol, 8.9-15.6 mg/L of naringenin, 0.1-7.7 mg/L of genistein, 0.9-4.6 mg/L of kaempferol and 0.26-0.38 mg/L of quercetin in defined media under optimal growth conditions. The recombinant yeast strains can be used further for the construction of improved flavonoid- and stilbenoid-overproducers.
由于植物次生代谢产物具有已证实或假设的有益特性和促进健康的作用，因此其化学或生物合成引起了越来越多的关注。白藜芦醇、柚皮素、染料木黄酮、山奈酚和槲皮素等都是具有高营养价值和农业价值的芪类、类黄酮和黄酮醇。已经构建了四个经过代谢工程改造的酵母菌株，这些菌株携带了质粒，其中包含了参与从苯丙氨酸生物合成这些化合物的酶的异源基因。通过对前体利用和终产物积累的时间进程分析，在最佳生长条件下，在限定培养基中生产了 0.29-0.31mg/L 的反式白藜芦醇、8.9-15.6mg/L 的柚皮素、0.1-7.7mg/L 的染料木黄酮、0.9-4.6mg/L 的山奈酚和 0.26-0.38mg/L 的槲皮素。这些重组酵母菌株可进一步用于构建改良的类黄酮和芪类过量产生菌。
