Fairchild Jacob N, Horváth Krisztián, Guiochon Georges
Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA.
J Chromatogr A. 2009 Aug 21;1216(34):6210-7. doi: 10.1016/j.chroma.2009.06.085. Epub 2009 Jul 7.
The theoretical advantages and drawbacks of using a multiple-, parallel column approach in on-line multidimensional liquid chromatography systems were investigated. Much time or peak capacity can be gained with the use of multiple parallel columns at the second-dimension while the aggregate time of separation increases only by the increment of the gradient time of the second-dimension. Multidimensional chromatographic systems are now used to perform many tasks ranging from routine, fast analyses to specialized, arduous separations. In this work, we focus on the advantages of a multiple, parallel columns approach to on-line multidimensional liquid chromatography systems. Calculations of the achievable peak capacities were made as functions of the number of columns operated in parallel. Increasing the number of second-dimension columns from one to two or three causes the largest increase in peak capacity with only a slight increase of aggregate time. We also present some practical aspects to consider when attempting multidimensional separations with multiple columns operated in parallel.
研究了在在线多维液相色谱系统中采用多平行柱方法的理论优缺点。在二维使用多个平行柱可以节省大量时间或增加峰容量，而总的分离时间仅随着二维梯度时间的增加而增加。多维色谱系统现在可用于执行许多任务，从常规的快速分析到专门的艰巨分离。在这项工作中，我们关注多平行柱方法用于在线多维液相色谱系统的优点。计算了可实现的峰容量与平行操作的柱数的函数关系。将二维柱的数量从一根增加到两根或三根会导致峰容量增加最多，而总时间仅略有增加。我们还介绍了在尝试使用多个平行操作的柱进行多维分离时需要考虑的一些实际问题。
