Gray M J, Slonecker P J, Dennis G, Shalliker R A
Nanoscale Organization and Dynamic Group, University of Western Sydney, Hawkesbury, Parramatta, NSW 1797, Australia.
J Chromatogr A. 2005 Nov 25;1096(1-2):92-100. doi: 10.1016/j.chroma.2005.06.091. Epub 2005 Jul 19.
The loading capacity of rod monolithic C18 columns was found to be sensitive to the injection volume, but essentially insensitive to the mass loading for a separation of oligostyrenes. When rod monoliths were coupled in series the injection volume loading increased, as too did the resolution of the oligomers, but at the expense of separation time. The volume load capacity of these serially connected monoliths was, however, not directly proportional to the number of columns connected. The volume load capacity was, however, directly proportional to the number of columns when the monoliths were connected in parallel and the flow stream split between each of the monolithic channels. When the number of monoliths in each channel equaled the number of monoliths that were connected in a single channel serial system the peak capacity and retention time was equivalent for both systems, but the volume load capacity of the parallel system was twice that of the serial connection each time the number of channels doubled. The results of this study indicate that parallel connection of rod monolithic columns may be useful for preparative scale and multidimensional separations where it is important that the volume load capacity is high.
发现棒状整体式C18柱的负载量对进样体积敏感，但对于寡聚苯乙烯的分离，对质量负载基本不敏感。当棒状整体柱串联连接时，进样体积负载增加，低聚物的分离度也增加，但以分离时间为代价。然而，这些串联连接的整体柱的体积负载量与连接的柱数不成正比。当整体柱并联连接且流路在每个整体通道之间分流时，体积负载量与柱数成正比。当每个通道中的整体柱数量等于在单通道串联系统中连接的整体柱数量时，两个系统的峰容量和保留时间相当，但每次通道数量翻倍时，并联系统的体积负载量是串联连接的两倍。本研究结果表明，棒状整体柱的并联连接对于制备规模和多维分离可能有用，在这些分离中，高体积负载量很重要。
