State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, No. 14, Sec. 3, Renminnan Road, Chengdu, Sichuan 610041, China.
Oral Oncol. 2009 Nov;45(11):e204-7. doi: 10.1016/j.oraloncology.2009.06.001. Epub 2009 Jul 23.
CAL 27 is one of the most frequently used cell lines in the field of oral squamous cell carcinoma (OSCC) studying. However, our recent studies showed that the growth pattern of CAL 27 xenograft did not seem like typical OSCC. In this study, we verified the identity of CAL 27 cells by using by short tandem repeat analysis. Then, we performed tumor formation assay, HE staining and immunohistochemistry assay to further study the growing characteristics and histopathological diagnosis of CAL 27 xenografts. Our results showed that CAL 27 xenografts grew slowly in vivo with vesicle formation at both the surfaces and deeper areas of the tumors. The CAL 27 xenografts were then diagnosed as oral adenosquamous carcinomas. Thus CAL 27 appears to be an oral adenosquamous carcinoma cell line.
CAL 27 是口腔鳞状细胞癌（OSCC）研究领域中最常使用的细胞系之一。然而，我们最近的研究表明，CAL 27 异种移植的生长模式似乎不像典型的 OSCC。在这项研究中，我们通过短串联重复分析验证了 CAL 27 细胞的身份。然后，我们进行了肿瘤形成测定、HE 染色和免疫组织化学测定，以进一步研究 CAL 27 异种移植的生长特征和组织病理学诊断。我们的结果表明，CAL 27 异种移植在体内生长缓慢，肿瘤表面和深层区域均形成囊泡。CAL 27 异种移植被诊断为口腔腺鳞癌。因此，CAL 27 似乎是一种口腔腺鳞癌细胞系。
