Institute of Bio-and Nanosystems, Research Center Juelich, Germany.
Bioelectrochemistry. 2010 Feb;77(2):89-93. doi: 10.1016/j.bioelechem.2009.06.015. Epub 2009 Jul 8.
In the present paper, we demonstrate the electrochemical rectification of a redox current which is transferred between redox probes (ferricyanide) in solution and a gold electrode functionalized with the biomolecular redox mediator microperoxidase-11 (MP-11). MP-11 is the redox active, heme-containing domain of the biological electron shuttle cytochrome c (cyt c). In our system, a unidirectional current develops due to selective electron transport from the bio-functionalized electrode to ferricyanide such that MP-11 controls the read-out of our coupled redox system. The electrode was functionalized by adding a monolayer of undecanethiol (UDT) to promote the physisorption of MP-11 and inhibit the direct electron transfer between redox probe and electrode. The relative position of redox donator, mediator, and acceptor equilibrium potentials defines the charge transport and a potential-dependent electrochemical current rectification. The results of our investigations demonstrate that functional building blocks of proteins can be reassembled into new conceptual devices with operation modes deviating from their native function, which could prove highly useful in future design of biosensors and bioelectronic systems.
在本文中，我们展示了在溶液中的氧化还原探针（铁氰化物）和功能化有生物分子氧化还原介体微过氧化物酶-11（MP-11）的金电极之间转移的氧化还原电流的电化学整流。MP-11 是生物电子穿梭体细胞色素 c（cyt c）的氧化还原活性血红素结构域。在我们的系统中，由于从生物功能化电极到铁氰化物的选择性电子传递，产生了单向电流，使得 MP-11 控制我们耦合的氧化还原系统的读出。通过添加单层十一烷硫醇（UDT）来促进 MP-11 的物理吸附并抑制氧化还原探针和电极之间的直接电子转移，从而对电极进行功能化。氧化还原给体、介体和受体平衡电位的相对位置决定了电荷传输和电位依赖的电化学电流整流。我们研究的结果表明，蛋白质的功能构建块可以重新组装成具有偏离其天然功能的操作模式的新概念设备，这在未来的生物传感器和生物电子系统设计中可能非常有用。
