Barker P D, Hill H A
Inorganic Chemistry Laboratory, Oxford University, UK.
Prog Clin Biol Res. 1988;274:419-33.
The direct electrochemistry of redox proteins has been achieved at a variety of electrodes, including modified gold and pyrolytic graphite. Careful design of electrode surfaces and electrolyte conditions are required for the attainment of rapid and reversible protein-electrode interaction. Protein-protein complexes can be studied by using selective electrode surfaces. The "well-behaved" electrochemistry of redox proteins can be exploited by coupling the electrode reaction to enzymes for which the redox proteins act as cofactors. The electron-transfer reactions of more complex systems, such as redox enzymes, are now being examined.
氧化还原蛋白的直接电化学已在包括修饰金电极和热解石墨电极在内的多种电极上得以实现。要实现快速且可逆的蛋白 - 电极相互作用，需要精心设计电极表面和电解质条件。通过使用选择性电极表面，可以研究蛋白质 - 蛋白质复合物。氧化还原蛋白“表现良好”的电化学性质可通过将电极反应与以氧化还原蛋白为辅因子的酶偶联来加以利用。目前正在研究更复杂体系的电子转移反应，如氧化还原酶的反应。
