Campos R, Garrido A, Guerra R, Valenzuela A
Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago.
Free Radic Res Commun. 1990;10(4-5):259-64. doi: 10.3109/10715769009149894.
Reperfusion of rat kidney submitted to temporal ischaemia induces a decrease in glutathione content. Lipid peroxidation is not detected in kidney homogenates but microsomes obtained after periods of reperfusion longer than 60 minutes show increased malondialdehyde values correlated with high oxygen consumption and superoxide free radical generation. Microsomes obtained from kidneys submitted to 15 or 60 minutes of reperfusion are resistant to NADPH-induced lipid peroxidation but after 120 minutes of reperfusion an increased lipid peroxidative response is observed. Although the mechanism of the protection found in microsomes against the induction of oxidative stress in the first 60 minutes of reperfusion is unknown, it is postulated that this subcellular fraction plays an important role in the oxidative stress observed after longer periods of reperfusion.
经历短暂缺血的大鼠肾脏再灌注会导致谷胱甘肽含量降低。在肾脏匀浆中未检测到脂质过氧化，但再灌注时间超过60分钟后获得的微粒体显示丙二醛值升高，这与高耗氧量和超氧自由基生成相关。经历15或60分钟再灌注的肾脏获得的微粒体对NADPH诱导的脂质过氧化具有抗性，但再灌注120分钟后，观察到脂质过氧化反应增强。尽管在再灌注的前60分钟微粒体中发现的保护机制尚不清楚，但据推测，这个亚细胞组分在再灌注较长时间后观察到的氧化应激中起重要作用。
