Solsona C, Egea G, Blasi J, Casanova C, Marsal J
Departament de Biologia Cellular i Anatomia Patológica, Facultat de Medicina, Hospital de Bellvitge, Universitat de Barcelona, c/, Spain.
J Physiol (Paris). 1990;84(2):174-9.
Botulinum neurotoxin type A (BoNTx) inhibits the release of acetylcholine (ACh) from Torpedo electric organ synaptosomes. We have studied several biochemical and morphological aspects in order to characterize the molecular interactions of BoNTx intoxication in our preparation. 1. We are describing for the first time an electrophoretic band from cholinergic presynaptic plasma membrane (PSPM) that is recognized by 125I-BoNTx as a putative BoNTx receptor. 2. Furthermore we describe direct interaction of botulinum toxin-gold complexes with synaptic vesicles through the three-step model of the BoNTx intoxication.
A型肉毒杆菌神经毒素（BoNTx）可抑制电鳐电器官突触体中乙酰胆碱（ACh）的释放。我们研究了几个生化和形态学方面的内容，以便在我们的制备物中表征BoNTx中毒的分子相互作用。1. 我们首次描述了胆碱能突触前质膜（PSPM）上的一条电泳带，它被125I-BoNTx识别为一种假定的BoNTx受体。2. 此外，我们通过BoNTx中毒的三步模型描述了肉毒杆菌毒素-金复合物与突触小泡的直接相互作用。
