去极化的电鳐突触体突触前膜中反应性胆固醇增加：A型肉毒杆菌毒素的阻断作用

Increase in reactive cholesterol in the presynaptic membrane of depolarized Torpedo synaptosomes: blockade by botulinum toxin type A.

作者信息

Egea G, Marsal J, Solsona C, Rabasseda X, Blasi J

机构信息

Departament de Biologia Cellular i Anatomia Patològica, Facultat de Medicina, Hospital de Bellvitge, Universitat de Barcelona, Barcelona, Spain.

出版信息

Neuroscience. 1989;31(2):521-7. doi: 10.1016/0306-4522(89)90394-1.

DOI:10.1016/0306-4522(89)90394-1

Abstract

We have investigated the redistribution of filipin-cholesterol complexes at freeze-fractured presynaptic membrane of pure cholinergic synaptosomes isolated from Torpedo electric organ during acetylcholine release. After chemical depolarization, filipin-induced lesions increase at the presynaptic membrane. These changes do not take place when synaptosomes are stimulated in a calcium-free medium. Botulinum neurotoxin type A blocks both acetylcholine release and the rearrangement of filipin-induced lesions induced by depolarization. Since botulinum neurotoxin type A does not block either membrane depolarization or calcium entry into the nerve terminal, our results suggest that the redistribution of filipin-cholesterol complexes is linked to the acetylcholine release process.

摘要

我们研究了在乙酰胆碱释放过程中，从电鳐电器官分离出的纯胆碱能突触体经冷冻断裂处理后的突触前膜上，制霉菌素-胆固醇复合物的重新分布情况。化学去极化后，突触前膜上制霉菌素诱导的损伤增加。当突触体在无钙培养基中受到刺激时，这些变化不会发生。A型肉毒杆菌神经毒素可阻断乙酰胆碱释放以及去极化诱导的制霉菌素损伤的重排。由于A型肉毒杆菌神经毒素既不阻断膜去极化，也不阻断钙进入神经末梢，我们的结果表明，制霉菌素-胆固醇复合物的重新分布与乙酰胆碱释放过程有关。

相似文献

1

Increase in reactive cholesterol in the presynaptic membrane of depolarized Torpedo synaptosomes: blockade by botulinum toxin type A.去极化的电鳐突触体突触前膜中反应性胆固醇增加：A型肉毒杆菌毒素的阻断作用

Neuroscience. 1989;31(2):521-7. doi: 10.1016/0306-4522(89)90394-1.

2

Botulinum toxin type A blocks the morphological changes induced by chemical stimulation on the presynaptic membrane of Torpedo synaptosomes.A型肉毒杆菌毒素可阻断化学刺激对电鳐突触体突触前膜诱导的形态学变化。

Proc Natl Acad Sci U S A. 1989 Jan;86(1):372-6. doi: 10.1073/pnas.86.1.372.

3

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4

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Cell Tissue Res. 1987 Apr;248(1):207-14. doi: 10.1007/BF01239982.

5

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6

Binding of botulinum neurotoxin to pure cholinergic nerve terminals isolated from the electric organ of Torpedo.肉毒杆菌神经毒素与从电鳐电器官分离出的纯胆碱能神经末梢的结合。

J Neural Transm Gen Sect. 1992;90(2):87-102. doi: 10.1007/BF01250791.

7

ATP release from pure cholinergic synaptosomes is not blocked by tetanus toxin.

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8

Energy metabolism and quantal acetylcholine release: effects of botulinum toxin, 1-fluoro-2,4-dinitrobenzene, and diamide in the Torpedo electric organ.能量代谢与量子化乙酰胆碱释放：肉毒杆菌毒素、1-氟-2,4-二硝基苯和二酰胺对电鳐电器官的影响

J Neurochem. 1988 Feb;50(2):431-9. doi: 10.1111/j.1471-4159.1988.tb02930.x.

9

The action of botulinum toxin on cholinergic nerve terminals isolated from the electric organ of Torpedo marmorata. Detection of a putative toxin receptor.肉毒杆菌毒素对从多纹电鳐电器官分离出的胆碱能神经末梢的作用。一种假定毒素受体的检测。

J Physiol (Paris). 1990;84(2):174-9.

10

Effect of cetiedil on acetylcholine release and intramembrane particles in cholinergic synaptosomes.

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