Department of Physics, McGill University, Montreal, Canada.
Cardiovasc Pathol. 2010 Jul-Aug;19(4):e125-8. doi: 10.1016/j.carpath.2009.06.001. Epub 2009 Jul 24.
We have used second-harmonic-generation (SHG) to image collagen fibers in pericardial tissue removed from a patient with constrictive pericarditis and compared this to healthy pericardium. SHG imaging allowed for the visualization of collagen fibers without the need for staining or pretreatment. Images were compared to stained histology slides. Collagen fibers in SHG and histology images displayed the same structure and morphology. The mature collagen of the parietal pericardium was easily distinguishable from the new collagen accumulation due to the pericarditis. SHG imaging can provide a convenient and valuable architectural profile of collagen organization.
我们使用二次谐波产生（SHG）技术对取自缩窄性心包炎患者的心包膜组织中的胶原纤维进行成像，并将其与健康的心包膜进行比较。SHG 成像允许在无需染色或预处理的情况下可视化胶原纤维。将图像与染色的组织学载玻片进行比较。SHG 和组织学图像中的胶原纤维显示出相同的结构和形态。由于心包炎，壁层心包的成熟胶原很容易与新的胶原积累区分开来。SHG 成像可以提供胶原组织的方便且有价值的结构轮廓。
