Laboratory for Photonics Research of Bio/nano Environments (PROBE), Department of Electrical and Computer Engineering,University of Illinois Urbana-Champaign, 1406 W Green Street, Urbana, Illinois 61801, USA.
Opt Lett. 2009 Dec 15;34(24):3779-81. doi: 10.1364/OL.34.003779.
Fourier transform second-harmonic generation (SHG) microscopy has been applied to quantitatively compare the information content between SHG images obtained from the forward and backward direction for three tissue types: porcine tendon, sclera, and ear cartilage. Both signal types yield consistent information on the preferred orientation of collagen fibers. For all specimens, the Fourier transform of the forward and backward SHG images produces several overlapping peaks in the magnitude spectrum at various depths into the tissues, indicating that some information present in the forward SHG images can be extracted from the backward SHG images. This study highlights the potential of backward SHG microscopy for medical diagnostics.
傅里叶变换二次谐波产生（SHG）显微镜已被应用于定量比较从三个组织类型的正向和反向方向获得的 SHG 图像之间的信息量：猪肌腱、巩膜和耳软骨。这两种信号类型都能提供关于胶原蛋白纤维优先取向的一致信息。对于所有标本，正向和反向 SHG 图像的傅里叶变换在组织内的不同深度产生了几个在幅度谱上重叠的峰，表明正向 SHG 图像中的一些信息可以从反向 SHG 图像中提取出来。这项研究强调了反向 SHG 显微镜在医学诊断中的潜力。
