Stanley F E, Warner A M, Gutierrez S M, Stalcup A M
University of Cincinnati, Department of Chemistry, OH 45221, USA.
Biochem Biophys Res Commun. 2009 Oct 9;388(1):28-30. doi: 10.1016/j.bbrc.2009.07.098. Epub 2009 Jul 24.
Circular dichroism (CD) and UV/Visible absorption (UV/Vis) spectroscopy techniques were used to investigate the interaction between heparin and chloroquine, an antimalarial drug that has shown potential as an anti-prion agent. CD spectra of rac-chloroquine upon addition of heparin provide evidence of glycosaminoglycan (GAG) binding, support recent findings suggesting that interactions between heparin and antimalarial drugs are largely due to electrostatic interactions, and represent the first reported GAG-induced CD signal of a bicyclic, aromatic compound. The association constant ( approximately 10(3)M(-1)) between chloroquine and heparin was calculated from a UV titration curve and provided additional insight into the nature of the association between these two compounds.
圆二色光谱（CD）和紫外/可见吸收光谱（UV/Vis）技术被用于研究肝素与氯喹之间的相互作用，氯喹是一种抗疟药物，已显示出作为抗朊病毒剂的潜力。添加肝素后消旋氯喹的CD光谱提供了糖胺聚糖（GAG）结合的证据，支持了最近的研究结果，即肝素与抗疟药物之间的相互作用在很大程度上是由于静电相互作用，并且代表了首次报道的双环芳香族化合物的GAG诱导的CD信号。从紫外滴定曲线计算出氯喹与肝素之间的缔合常数（约10³ M⁻¹），这为深入了解这两种化合物之间缔合的性质提供了更多信息。
