Department of Chemical Engineering, Queen's University, Kingston, Ontario K7L3N6, Canada.
J Biotechnol. 2009 Sep 25;143(4):262-7. doi: 10.1016/j.jbiotec.2009.07.014. Epub 2009 Jul 24.
Medium-chain-length polyhydroxyalkanoates (MCL-PHAs) were produced in carbon-limited, single-stage, fed-batch fermentations of Pseudomonas putida KT2440 by co-feeding nonanoic acid (NA) and glucose (G) to enhance the yield of PHA from NA. An exponential (mu=0.25 h(-1)) followed by a linear feeding strategy at a NA:G ratio of 1:1 (w/w) achieved 71 g l(-1) biomass containing 56% PHA. Although the same overall PHA productivity (1.44 g l(-1) h(-1)) was obtained when NA alone was fed at the same specific growth rate, the overall yield of PHA from NA increased by 25% (0.66 g PHA g NA(-1) versus 0.53 g g(-1)) with glucose co-feeding. Further increasing glucose in the feed (NA:G=1:1.5) resulted in a slightly higher yield (0.69 g PHA g NA(-1)) but lower PHA content (48%) and productivity (1.16 g l(-1) h(-1)). There was very little change in the PHA composition.
中链长度聚羟基烷酸酯（MCL-PHAs）在假单胞菌 KT2440 的碳限制、单级、补料分批发酵中生产，通过共补加壬酸（NA）和葡萄糖（G）来提高从 NA 生产 PHA 的产率。采用指数（μ=0.25 h（-1））随后以 NA:G 比为 1:1（w/w）的线性补料策略，实现了 71 g l（-1）生物量，其中含有 56%的 PHA。尽管以相同的比生长速率单独补加 NA 时获得了相同的总 PHA 生产力（1.44 g l（-1）h（-1）），但与葡萄糖共补料时，从 NA 生产 PHA 的总产率提高了 25%（0.66 g PHA g NA（-1）对 0.53 g g（-1））。进一步增加进料中的葡萄糖（NA:G=1:1.5）会导致产率略有提高（0.69 g PHA g NA（-1）），但 PHA 含量（48%）和生产力（1.16 g l（-1）h（-1））降低。PHA 组成几乎没有变化。
