Chemical Engineering, Queen's University, Kingston, ON K7L 3N6, Canada.
AMB Express. 2013 Aug 29;3(1):50. doi: 10.1186/2191-0855-3-50.
With no inhibition of β-oxidation, Pseudomonas putida KT2440 produces medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA) with approximately 65 mol% 3-hydroxynonanoate (HN) from nonanoic acid. Production of PHA with higher HN content and an adjustable monomeric composition was obtained using acrylic acid, a fatty acid β-oxidation inhibitor, together with nonanoic acid and glucose as co-substrates in fed-batch fermentations. Different monomeric compositions were obtained by varying the feeding conditions to impose different specific growth rates and inhibitor feed concentrations. At a nonanoic acid: glucose: acrylic acid feed mass ratio of 1.25: 1: 0.05 and a specific growth rate of 0.15 h-1, 71.4 g L-1 biomass was produced containing 75.5% PHA with 89 mol% HN at a cumulative PHA productivity of 1.8 g L-1 h-1.
在没有β-氧化抑制的情况下，恶臭假单胞菌 KT2440 可以利用壬酸生产出约 65 mol% 3-羟基壬酸（HN）的中链长度聚（3-羟基烷酸酯）（MCL-PHA）。在分批补料发酵中，使用丙烯酸（一种脂肪酸β-氧化抑制剂）与壬酸和葡萄糖作为共底物，可以生产出具有更高 HN 含量和可调节单体组成的 PHA。通过改变进料条件来施加不同的比生长速率和抑制剂进料浓度，可以获得不同的单体组成。在壬酸：葡萄糖：丙烯酸进料质量比为 1.25:1:0.05，比生长速率为 0.15 h-1 的条件下，生产出了 71.4 g/L 的生物量，其中 75.5%为 PHA，89 mol%为 HN，PHA 累积产率为 1.8 g/L·h-1。
