Department of Kampo Diagnostics, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Evid Based Complement Alternat Med. 2011;2011:914249. doi: 10.1093/ecam/nep089. Epub 2011 May 3.
The effects of keishibukuryogan on the early stage of progressive renal failure were examined in rats subjected to 5/6 nephrectomy. Keishibukuryogan, one of the traditional herbal formulations, was given orally at a dose of 1% (w/w) and 3% (w/w) in chow. Administration of keishibukuryogan was started at 1 week after 5/6 nephrectomy and was continued for 4 weeks. At the end of the experiment, Azan staining did not reveal any severe histological changes in the kidneys of the nephrectomized rats. On the other hand, significant increases in mRNA expressions of transforming growth factor-β(1) and fibronectin related to tissue fibrosis, as examined by Reverse Transcriptase-Polymerase Chain Reaction, were observed in nephrectomized rats, and they were significantly suppressed by 3% keishibukuryogan treatment. Against gene expressions related to macrophage infiltration, 3% keishibukuryogan treatment significantly suppressed osteopontin mRNA levels, and monocyte chemoattractant protein-1 and vascular cell adhesion molecule-1 mRNA levels showed a tendency to decrease, but without statistical significance. It was also observed that 3% keishibukuryogan attenuated serum urea nitrogen and urinary protein excretion levels. From these results, it was suggested that keishibukuryogan exerts beneficial effects that result in slowing the progression of chronic renal failure.
观察了方剂“keishibukuryogan”对 5/6 肾切除大鼠早期进行性肾衰竭的影响。keishibukuryogan 是一种传统草药配方，以 1%（w/w）和 3%（w/w）的剂量添加到饲料中进行口服给药。给药在 5/6 肾切除术后 1 周开始，并持续 4 周。实验结束时，阿赞染色未显示肾切除大鼠肾脏有任何严重的组织学变化。另一方面，通过逆转录聚合酶链反应（Reverse Transcriptase-Polymerase Chain Reaction）检查到，纤维化相关的转化生长因子-β(1)和纤维连接蛋白的 mRNA 表达在肾切除大鼠中显著增加，而 3%的 keishibukuryogan 处理显著抑制了这些增加。针对与巨噬细胞浸润相关的基因表达，3%的 keishibukuryogan 处理显著抑制了骨桥蛋白 mRNA 水平，单核细胞趋化蛋白-1 和血管细胞黏附分子-1 mRNA 水平也表现出降低的趋势，但无统计学意义。还观察到 3%的 keishibukuryogan 降低了血清尿素氮和尿蛋白排泄水平。从这些结果表明，方剂“keishibukuryogan”具有有益的作用，可以减缓慢性肾衰竭的进展。
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