Goto H, Shimada Y, Sekiya N, Yang Q, Kogure T, Mantani N, Hikiami H, Shibahara N, Terasawa K
Faculty of Medicine, and Department of Kampo Diagnostics, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Japan.
Phytomedicine. 2004 Feb;11(2-3):188-95. doi: 10.1078/0944-7113-00336.
Keishi-bukuryo-gan (Gui-zhi-fu-ling-wan) is a formula used for the improvement of blood circulation. Recently it has often also been used for arteriosclerosis. One of the mechanisms involved is thought to be the improvement of endothelial dysfunction, but the details are still unclear. In this study, the effect of Keishi-bukuryo-gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats was studied. Rats were given Keishi-bukuryo-gan in chow for 30 weeks. Body weight, blood glucose, endothelium-dependent/-independent relaxation, vasocontraction by free radical-induced and contractive prostanoids, triglyceride, advanced glycation endproduct, lipid peroxides, serum NO2-/NO3- and blood viscosity were measured. The results indicated that Keishi-bukuryo-gan caused a decrease in endothelium-dependent relaxation by acetylcholine to become significantly increased, and vasocontraction induced by free radicals and contractive prostanoids was significantly decreased. Furthermore, serum NO2-/NO3- and blood viscosity were significantly decreased. From these results, it was supposed that Keishi-bukuryo-gan exerted a protective effect on the endothelium. The WBN/kob rat is a useful study model for the complications of human diabetes, and Keishi-bukuryo-gan showed a protective effect against vascular injury in the susceptible rat.
桂枝茯苓丸是一种用于改善血液循环的方剂。最近它也常常被用于治疗动脉硬化。其涉及的机制之一被认为是改善内皮功能障碍，但具体细节仍不清楚。在本研究中，研究了桂枝茯苓丸对自发性糖尿病（WBN/kob）大鼠血管功能和血液流变学因素的影响。给大鼠在饲料中添加桂枝茯苓丸，持续30周。测量体重、血糖、内皮依赖性/非依赖性舒张、自由基诱导的血管收缩和收缩性前列腺素、甘油三酯、晚期糖基化终产物、脂质过氧化物、血清NO2-/NO3-和血液粘度。结果表明，桂枝茯苓丸使乙酰胆碱诱导的内皮依赖性舒张显著增加，自由基和收缩性前列腺素诱导的血管收缩显著降低。此外，血清NO2-/NO3-和血液粘度显著降低。从这些结果推测，桂枝茯苓丸对内皮具有保护作用。WBN/kob大鼠是研究人类糖尿病并发症的有用模型，桂枝茯苓丸对易患大鼠的血管损伤具有保护作用。
