Jay D, Zamorano R, Muñoz E, Gleason R, Boldu J L
Departamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, México, D.F.
Bol Estud Med Biol. 1990 Jan-Jun;38(1-2):16-21.
The EPR spectra of phenazine methosulfate (PMS) generated under different conditions, as reduction or excitation with light were studied. In addition, results show the EPR spectra of reduced-submitochondrial particles and reduced-submitochondrial particles in the presence of PMS. Combined systems of this last type have been repeatedly utilized in EPR studies. This work proves that such systems give rise to characteristic signals around g = 2, which behaviour reflects the presence of particular prosthetic groups of the respiratory chain combined with the reduced dye. The consequences of these findings are discussed.
研究了在不同条件下生成的吩嗪硫酸甲酯（PMS）的电子顺磁共振（EPR）光谱，如还原或光照激发条件下的光谱。此外，结果还展示了还原型亚线粒体颗粒以及存在PMS时还原型亚线粒体颗粒的EPR光谱。后一种类型的组合系统已在EPR研究中多次使用。这项工作证明，此类系统在g = 2附近会产生特征信号，其行为反映了呼吸链特定辅基与还原型染料结合的存在情况。讨论了这些发现的影响。
