Pincus S M, Beckman B S, Broadway R L, George W J, Jeter J R
Department of Anatomy, Tulane Medical School, New Orleans, Louisiana 70112.
Cytobios. 1990;64(256):23-33.
Immediate changes in protein phosphorylation in Friend erythroleukaemia cells (FELC) were examined in response to stimulation with dimethylsulphoxide (DMSO), which triggers cell differentiation, 12-O-tetra-decanoyl-phorbol 13-acetate (TPA), a tumour promoter, and the Ca++ ionophore A23187. The effects of the cyclic nucleotides, cAMP and cGMP, on the patterns of phosphoproteins were also analysed. Autoradiographs of two-dimensional gels reveal that within 30 min of treatment by the various agents differences in the patterns of protein phosphorylation can be seen. Treatment with DMSO and TPA altered the phosphoprotein patterns in different ways. These patterns were distinct from those observed in untreated and cyclic nucleotide treated cells. Treatment with the calcium ionophore caused a unique phosphorylation pattern unlike any of the others. These data suggest that the very early changes seen in the patterns of protein phosphorylation in FELC may be related to the induction of differentiation.
研究了弗氏红白血病细胞（FELC）中蛋白质磷酸化的即时变化，以响应二甲基亚砜（DMSO）、12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA，一种肿瘤促进剂）和钙离子载体A23187的刺激，其中DMSO可触发细胞分化。还分析了环核苷酸cAMP和cGMP对磷蛋白模式的影响。二维凝胶放射自显影片显示，在各种试剂处理30分钟内，可观察到蛋白质磷酸化模式的差异。用DMSO和TPA处理以不同方式改变了磷蛋白模式。这些模式与未处理细胞和经环核苷酸处理的细胞中观察到的模式不同。用钙离子载体处理导致了一种独特的磷酸化模式，与其他任何模式都不同。这些数据表明，在FELC中蛋白质磷酸化模式中看到的非常早期的变化可能与分化诱导有关。
