Hösli E, Hösli L
Department of Physiology, University of Basel, Switzerland.
Exp Brain Res. 1990;82(3):667-71. doi: 10.1007/BF00228810.
Explant cultures of rat spinal cord, brain stem and cerebellum were used to visualize GABAA-receptors by means of immunohistochemistry. For these studies we have incubated the cultures with the monoclonal antibody bd 17 against the beta-subunit of the GABAA/benzodiazepine/chloride channel complex. In spinal cord cultures, many interneurones were immunoreactive whereas only a small number of large neurones, probably motoneurones was specifically stained. In brain stem cultures, groups of large and medium-sized neurones showed immunoreactivity. In cultures of cerebellum, a great number of neurones was specifically stained. Granule cells showed the strongest immunoreactivity whereas other neurones, presumably Purkinje cells and interneurones, were only moderately stained. The immunoreactivity was mainly confined to the cell bodies of the neurones while their processes were only weakly or not stained. In contrast to neurones, no immunoreactivity could be detected on astrocytes.
利用大鼠脊髓、脑干和小脑的外植体培养物，通过免疫组织化学方法来观察γ-氨基丁酸A型（GABAA）受体。在这些研究中，我们用针对GABAA/苯二氮䓬/氯化物通道复合物β亚基的单克隆抗体bd 17孵育培养物。在脊髓培养物中，许多中间神经元具有免疫反应性，而只有少数大神经元（可能是运动神经元）被特异性染色。在脑干培养物中，成组的大中型神经元显示出免疫反应性。在小脑培养物中，大量神经元被特异性染色。颗粒细胞显示出最强的免疫反应性，而其他神经元（可能是浦肯野细胞和中间神经元）仅被适度染色。免疫反应性主要局限于神经元的细胞体，而它们的突起仅被弱染色或未被染色。与神经元不同，在星形胶质细胞上未检测到免疫反应性。
