Shivers B D, Killisch I, Sprengel R, Sontheimer H, Köhler M, Schofield P R, Seeburg P H
Center for Molecular Biology, Federal Republic of Germany.
Neuron. 1989 Sep;3(3):327-37. doi: 10.1016/0896-6273(89)90257-2.
Two cDNAs encoding novel GABAA receptor subunits were isolated from a rat brain library. These subunits, gamma 2 and delta, share approximately 35% sequence identity with alpha and beta subunits and form functional GABA-gated chloride channels when expressed alone in vitro. The gamma 2 subunit is the rat homolog of the human gamma 2 subunit recently shown to be important for benzodiazepine pharmacology. Cellular localization of the mRNAs encoding the gamma 2 and delta subunits in rat brain revealed that largely distinct neuronal subpopulations express the two subunits. The delta subunit distribution resembles that of the high affinity GABAA receptor labeled with [3H]muscimol; the gamma 2 subunit distribution resembles that of GABAA/benzodiazepine receptors labeled with [3H]flunitrazepam. These findings have implications for the composition of two different GABAA receptor subtypes and for information processing in networks using GABA for signaling.
从大鼠脑文库中分离出两个编码新型GABAA受体亚基的cDNA。这些亚基，γ2和δ，与α和β亚基具有约35%的序列同一性，并且当在体外单独表达时形成功能性GABA门控氯离子通道。γ2亚基是最近显示对苯二氮䓬药理学很重要的人γ2亚基的大鼠同源物。大鼠脑中编码γ2和δ亚基的mRNA的细胞定位表明，在很大程度上不同的神经元亚群表达这两个亚基。δ亚基的分布类似于用[3H]蝇蕈醇标记的高亲和力GABAA受体的分布；γ2亚基的分布类似于用[3H]氟硝西泮标记的GABAA/苯二氮䓬受体的分布。这些发现对两种不同GABAA受体亚型的组成以及对使用GABA进行信号传导的网络中的信息处理具有启示意义。
